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Literature summary for 3.2.1.54 extracted from
- Characterization of thermostable cyclodextrinase from Clostridium thermohydrosulfuricum 39E (1990), Appl. Environ. Microbiol., 9, 2941-2943.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | - |
Thermoanaerobacter thermohydrosulfuricus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter thermohydrosulfuricus | - |
39E | - |
| Thermoanaerobacter thermohydrosulfuricus 39E | - |
39E | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified 205fold | Thermoanaerobacter thermohydrosulfuricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.5 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + H2O | 53% hydrolysis | Thermoanaerobacter thermohydrosulfuricus | ? | - |
? | |
| amylose + H2O | 53% hydrolysis | Thermoanaerobacter thermohydrosulfuricus 39E | ? | - |
? | |
| beta-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | glucose + maltose + long chain linear oligomers | - |
? | |
| beta-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | glucose + maltose + long chain linear oligomers | - |
? | |
| cyclomaltodextrin + H2O | alpha-cyclodextrin 100% hydrolysis, beta-cyclodextrin 67% hydrolysis, gamma-cyclodextrin 8% | Thermoanaerobacter thermohydrosulfuricus | maltodextrin | - |
? | |
| cyclomaltodextrin + H2O | alpha-cyclodextrin 100% hydrolysis, beta-cyclodextrin 67% hydrolysis, gamma-cyclodextrin 8% | Thermoanaerobacter thermohydrosulfuricus 39E | maltodextrin | - |
? | |
| starch + H2O | 50% hydrolysis | Thermoanaerobacter thermohydrosulfuricus | glucose + maltose | - |
? | |
| starch + H2O | 50% hydrolysis | Thermoanaerobacter thermohydrosulfuricus 39E | glucose + maltose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
beta-cyclodextrin as substrate | Thermoanaerobacter thermohydrosulfuricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | - |
Thermoanaerobacter thermohydrosulfuricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | enzyme half-life 402 min at 60°C, 180 min at 65°C, 75 min at 70°C | Thermoanaerobacter thermohydrosulfuricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Thermoanaerobacter thermohydrosulfuricus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
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