Literature summary for 3.2.1.52 extracted from

Tropak, M.B.; Zhang, J.; Yonekawa, S.; Rigat, B.A.; Aulakh, V.S.; Smith, M.R.; Hwang, H.J.; Ciufolini, M.A.; Mahuran, D.J.
Pyrimethamine derivatives insight into binding mechanism and improved enhancement of mutant beta-N-acetylhexosaminidase activity (2015), J. Med. Chem., 58, 4483-4493 .

Search for more literature for 3.2.1.52

Inhibitors

Inhibitors	Comment	Organism	Structure
2-amino-4-ethyl-5-(4-(trifluoromethyl)phenyl)pyrimidine	-	Homo sapiens
2-amino-4-ethyl-5-(4-chlorophenyl)pyrimidine	-	Homo sapiens
2-amino-6-ethyl-5-(4-chlorophenyl)pyrimidin-4(3H)-one	-	Homo sapiens
4-ethyl-5-[4-(trifluoromethyl)phenyl]pyrimidine	-	Homo sapiens
5-(3,5-dimethylphenyl)-6-ethylpyrimidine-2,4-diamine	-	Homo sapiens
5-(4-chlorophenyl)-6-butylpyrimidine-2,4-diamine	-	Homo sapiens
5-(4-chlorophenyl)-6-isopropylpyrimidine-2,4-diamine	-	Homo sapiens
5-(4-chlorophenyl)-6-methylpyrimidine-2,4-diamine	-	Homo sapiens
5-(4-chlorophenyl)-6-propylpyrimidine-2,4-diamine	-	Homo sapiens
6-(aminomethyl)-5-(4-chlorophenyl)pyrimidine-2,4-diamine	-	Homo sapiens
6-ethyl-5-(4-(trifluoromethyl)phenyl)pyrimidine-2,4-diamine	-	Homo sapiens
6-ethyl-5-(4-methoxyphenyl)pyrimidine-2,4-diamine	-	Homo sapiens
6-ethyl-5-(m-tolyl)pyrimidine-2,4-diamine	-	Homo sapiens
6-ethyl-5-(o-tolyl)pyrimidine-2,4-diamine	-	Homo sapiens
6-ethyl-5-(p-tolyl)pyrimidine-2,4-diamine	-	Homo sapiens
6-ethyl-5-phenylpyrimidine-2,4-diamine	-	Homo sapiens
pyrimethamine	i.e. 5-(4-chlorophenyl)-6-ethylpyrimidine-2,4-diamine	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Homo sapiens	5764	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ganglioside GM2 + H2O	Homo sapiens	-	ganglioside GM3 + N-acetyl-beta-D-galactosamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
fibroblast	-	Homo sapiens	-
placenta	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside 6-sulfate + H2O	-	Homo sapiens	4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside 6-sulfate	-	?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O	-	Homo sapiens	4-nitrophenol + N-acetyl-beta-D-glucosamine	-	?
ganglioside GM2 + H2O	-	Homo sapiens	ganglioside GM3 + N-acetyl-beta-D-galactosamine	-	?

Subunits

Subunits	Comment	Organism
heterodimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
beta-N-acetylhexosaminidase A	-	Homo sapiens
beta-N-acetylhexosaminidase B	-	Homo sapiens
Hex A	-	Homo sapiens
Hex B	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0031	-	wild type enzyme isoform Hex A from placenta, at pH 7.0 and 37°C	Homo sapiens	pyrimethamine
0.0045	-	wild type enzyme isoform Hex B from placenta, at pH 7.0 and 37°C	Homo sapiens	pyrimethamine
0.0085	-	mutant enzyme G269S of isoform Hex A from fibroblasts, at pH 4.5 and 37°C	Homo sapiens	pyrimethamine
0.0089	-	wild type enzyme isoform Hex A from placenta, at pH 4.5 and 37°C	Homo sapiens	pyrimethamine
0.0091	-	wild type enzyme isoform Hex B from placenta, at pH 4.5 and 37°C	Homo sapiens	pyrimethamine
0.014	-	wild type enzyme isoform Hex A from fibroblasts, at pH 4.5 and 37°C	Homo sapiens	pyrimethamine

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Release 2023.1 (January 2023)