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Literature summary for 3.2.1.52 extracted from

  • Jamek, S.B.; Muschiol, J.; Holck, J.; Zeuner, B.; Busk, P.K.; Mikkelsen, J.D.; Meyer, A.S.
    Loop protein engineering for improved transglycosylation activity of a beta-N-acetylhexosaminidase (2018), ChemBioChem, 19, 1858-1865 .
    View publication on PubMed
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli T7 Express lysY cells uncultured Bacteroidetes bacterium
expressed in Escherichia coli T7 Express lysY cells uncultured Actinomycetales bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Actinomycetales bacterium
0.119
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Bacteroidetes bacterium

Organism

Organism UniProt Comment Textmining
uncultured Actinomycetales bacterium A0A0E3XI19
-
-
uncultured Bacteroidetes bacterium A0A0E3XHF8
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography uncultured Bacteroidetes bacterium
His-Trap column chromatography uncultured Actinomycetales bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 uncultured Bacteroidetes bacterium 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 uncultured Actinomycetales bacterium 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
N,N'-diacetylchitobiose + beta-lactose transglycosylation uncultured Bacteroidetes bacterium lacto-N-triose II + N-acetyl-beta-D-glucosamine
-
 ?
N,N'-diacetylchitobiose + beta-lactose transglycosylation uncultured Actinomycetales bacterium lacto-N-triose II + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE uncultured Actinomycetales bacterium
? x * 60000, SDS-PAGE uncultured Bacteroidetes bacterium

Synonyms

Synonyms Comment Organism
Hex1
-
 uncultured Actinomycetales bacterium
Hex2
-
 uncultured Bacteroidetes bacterium
hexosaminidase
-
 uncultured Bacteroidetes bacterium
hexosaminidase
-
 uncultured Actinomycetales bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.6
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Bacteroidetes bacterium
19.4
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Actinomycetales bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
96.6
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Bacteroidetes bacterium
460
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Actinomycetales bacterium