Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.52 extracted from

  • Jiang, S.; Jiang, H.; Zhou, Y.; Jiang, S.; Zhang, G.
    High-level expression of beta-N-acetylglucosaminidase BsNagZ in Pichia pastoris to obtain GlcNAc (2019), Bioprocess Biosyst. Eng., 42, 611-619 .
    View publication on PubMed
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X33 Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Bacillus subtilis
-
 N-acetyl-beta-D-glucosamine
-
 ?
chitin + H2O Bacillus subtilis 168
-
 N-acetyl-beta-D-glucosamine
-
 ?
chitosan + H2O Bacillus subtilis
-
 N-acetyl-beta-D-glucosamine
-
 ?
chitosan + H2O Bacillus subtilis 168
-
 N-acetyl-beta-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P40406
-
-
Bacillus subtilis 168 P40406
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Bacillus subtilis 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Bacillus subtilis 168 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
chitin + H2O
-
 Bacillus subtilis N-acetyl-beta-D-glucosamine
-
 ?
chitin + H2O
-
 Bacillus subtilis 168 N-acetyl-beta-D-glucosamine
-
 ?
chitosan + H2O
-
 Bacillus subtilis N-acetyl-beta-D-glucosamine
-
 ?
chitosan + H2O
-
 Bacillus subtilis 168 N-acetyl-beta-D-glucosamine
-
 ?
colloidal chitin + H2O
-
 Bacillus subtilis N-acetyl-beta-D-glucosamine + ?
-
 ?
colloidal chitin + H2O
-
 Bacillus subtilis 168 N-acetyl-beta-D-glucosamine + ?
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Bacillus subtilis N-acetyl-beta-D-glucosamine
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Bacillus subtilis 168 N-acetyl-beta-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
beta-N-acetylglucosaminidase
-
 Bacillus subtilis
NAGase
-
 Bacillus subtilis
NagZ
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 65 the enzyme maintains over 60% relative activity from 45 to 65°C Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 60 the enzyme keeps over 80% activity after pre-incubation at 55°C for 1 h and 60% residual activity after 10 min pre-incubation at 60°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 the enzyme remains over 60% relative activity from pH 5.5-7.0 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10 the enzyme keeps over 80% activity after pre-incubation at 4°C for 12 h from pH 4.5-10.0 Bacillus subtilis