Literature summary for 3.2.1.52 extracted from

Gomes Junior, J.E.; Souza, D.S.; Nascimento, R.M.; Lima, A.L.; Melo, J.A.; Rocha, T.L.; Miller, R.N.; Franco, O.L.; Grossi-de-Sa, M.F.; Abreu, L.R.
Purification and characterization of a liver-derived beta-N-acetylhexosaminidase from marine mammal Sotalia fluviatilis (2010), Protein J., 29, 188-194.

Search for more literature for 3.2.1.52

Activating Compound

Activating Compound	Comment	Organism	Structure
chondroitin 6-sulfate	51% activation at 3 mM	Sotalia fluviatilis
D-fructose	58% activation at 3 mM	Sotalia fluviatilis
D-galactose	80% activation at 3 mM	Sotalia fluviatilis
D-glucose	64% activation at 3 mM	Sotalia fluviatilis
D-glucuronic acid	65% activation at 3 mM	Sotalia fluviatilis
diethyldicarbonate	37% activation at 1 mM	Sotalia fluviatilis
heparin	60% activation at 3 mM	Sotalia fluviatilis
lactose	71% activation at 3 mM	Sotalia fluviatilis
maltose	56% activation at 3 mM	Sotalia fluviatilis
N-acetylgalactosamine	9% activation at 3 mM	Sotalia fluviatilis
N-acetylglucosamine	38% activation at 3 mM	Sotalia fluviatilis
N-ethylmaleimide	38% activation at 1 mM	Sotalia fluviatilis
PMSF	74% activation at 1 mM	Sotalia fluviatilis
sucrose	44% activation at 3 mM	Sotalia fluviatilis

Inhibitors

Inhibitors	Comment	Organism	Structure
fucoidan	88% inhibition at 3 mM	Sotalia fluviatilis
HgCl2	70% inhibition at 1 mM	Sotalia fluviatilis
Na2S2O3	9% inhibition at 1 mM	Sotalia fluviatilis
SDS	21% inhibition at 1 mM	Sotalia fluviatilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ag+	activates 15% at 1 mM	Sotalia fluviatilis
Ba2+	activates 22% at 1 mM	Sotalia fluviatilis
Ca2+	activates 22% at 1 mM	Sotalia fluviatilis
Cu2+	activates 13% at 1 mM	Sotalia fluviatilis
Li+	activates 20% at 1 mM	Sotalia fluviatilis
additional information	no effect by 1 mM iodoacetamide	Sotalia fluviatilis
Na2SeO4	activates 16% at 1 mM	Sotalia fluviatilis
phosphate	activates 17% at 1 mM	Sotalia fluviatilis
Zn2+	activates 33% at 1 mM	Sotalia fluviatilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
10077	-	x * 10077, SDS-PAGE and mass spectrometry	Sotalia fluviatilis

Organism

Organism	UniProt	Comment	Textmining
Sotalia fluviatilis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 380.5fold from liver by ammonium sulfate fractionation, by gel filtration, and by chitin affinity, anion exchange, and hydroxy apatite chromatography	Sotalia fluviatilis

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Sotalia fluviatilis	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
22.25	-	purified native enzyme	Sotalia fluviatilis

Subunits

Subunits	Comment	Organism
?	x * 10077, SDS-PAGE and mass spectrometry	Sotalia fluviatilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Sotalia fluviatilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Sotalia fluviatilis

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	8.5	-	Sotalia fluviatilis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)