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Literature summary for 3.2.1.52 extracted from

  • Rattanakit, N.; Yano, S.; Plikomol, A.; Wakayama, M.; Tachiki, T.
    Purification of Aspergillus sp. S1-13 chitinases and their role in saccharification of chitin in mash of solid-state culture with shellfish waste (2007), J. Biosci. Bioeng., 103, 535-541.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
GlcNAc exochitinase Aspergillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
1 * 73000, exochitinase, SDS-PAGE Aspergillus sp.

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
S1-13
-
Aspergillus sp. S1-13
-
S1-13
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
-
Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information exochitinase is not active with colloidal chitin, powder chitin and lactic acid-treated crab shell Aspergillus sp. ?
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
?
p-nitrophenyl-GlcNAc + H2O exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
?
p-nitrophenyl-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
?
p-nitrophenyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O best substrate, exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
?

Subunits

Subunits Comment Organism
monomer 1 * 73000, exochitinase, SDS-PAGE Aspergillus sp.

Synonyms

Synonyms Comment Organism
exochitinase
-
Aspergillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
exochitinase Aspergillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH 5.5, 10 min, exochitinase, no loss of activity Aspergillus sp.
70
-
pH 5.5, 10 min, exochitinase, 50% loss of activity Aspergillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 exochitinase Aspergillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6.5 stable, exochitinase Aspergillus sp.