Protein Variants | Comment | Organism |
---|---|---|
D183N | no activity with 4-methylumbelliferyl N-acetyl-beta-D-glucosamine. Kcat/KM for 4-nitrophenyl N-acetyl-beta-D-glucosamine is 13333fold lower than wild-type value. Biofilm-detachment activity is very low | Aggregatibacter actinomycetemcomitans |
E184Q | no activity with 4-methylumbelliferyl N-acetyl-beta-D-glucosamine. Kcat/KM for 4-nitrophenyl N-acetyl-beta-D-glucosamine is 70.6fold lower than wild-type value. pH-optimum is shifted from pH 5.8 for wild-type enzyme to pH 6.8 for the mutant enzyme | Aggregatibacter actinomycetemcomitans |
E332Q | no activity with 4-methylumbelliferyl N-acetyl-beta-D-glucosamine. Kcat/KM for 4-nitrophenyl N-acetyl-beta-D-glucosamine is 2000fold lower than wild-type value. Biofilm-detachment activity is 28fold less efficient than the wild-type | Aggregatibacter actinomycetemcomitans |
R27A | no activity with 4-methylumbelliferyl N-acetyl-beta-D-glucosamine. Kcat/KM for 4-nitrophenyl N-acetyl-beta-D-glucosamine is 2400fold lower than wild-type value. Biofilm-detachment activity is 20fold less efficient than the wild-type | Aggregatibacter actinomycetemcomitans |
R27K | no activity with 4-methylumbelliferyl N-acetyl-beta-D-glucosamine. Kcat/KM for 4-nitrophenyl N-acetyl-beta-D-glucosamine is 1714fold lower than wild-type value. Biofilm-detachment activity is 26fold less efficient than the wild-type | Aggregatibacter actinomycetemcomitans |
W237A | no activity with 4-methylumbelliferyl N-acetyl-beta-D-glucosamine and 4-nitrophenyl N-acetyl-beta-D-glucosamine. Biofilm-detachment activity is 167fold less efficient than the wild-type | Aggregatibacter actinomycetemcomitans |
W330Y | no activity with 4-methylumbelliferyl N-acetyl-beta-D-glucosamine and 4-nitrophenyl N-acetyl-beta-D-glucosamine. Biofilm-detachment activity is 19.2fold less efficient than the wild-type | Aggregatibacter actinomycetemcomitans |
Y187A | Kcat/KM for 4-nitrophenyl N-acetyl-beta-D-glucosamine is 40fold lower than wild-type value. Kcat/KM for 4-methylumbelliferyl N-acetyl-beta-D-glucosamine is 4.9fold lower than wild-type value. Biofilm-detachment activity is 8.3fold less efficient than the wild-type | Aggregatibacter actinomycetemcomitans |
Y278A | Kcat/KM for 4-nitrophenyl N-acetyl-beta-D-glucosamine is 923fold lower than wild-type value. Kcat/KM for 4-methylumbelliferyl N-acetyl-beta-D-glucosamine is 45fold lower than wild-type value. Biofilm-detachment activity is 11fold less efficient than the wild-type | Aggregatibacter actinomycetemcomitans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAG-thiazoline | mechanism-based inhibitor | Aggregatibacter actinomycetemcomitans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, wild-type enzyme | Aggregatibacter actinomycetemcomitans | |
0.59 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme Y187A | Aggregatibacter actinomycetemcomitans | |
1.13 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme Y278A | Aggregatibacter actinomycetemcomitans | |
3.7 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme E184Q | Aggregatibacter actinomycetemcomitans | |
6.5 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme E332Q | Aggregatibacter actinomycetemcomitans | |
14.5 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme R27A | Aggregatibacter actinomycetemcomitans | |
19.4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme D183N | Aggregatibacter actinomycetemcomitans | |
35.9 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme Y278A | Aggregatibacter actinomycetemcomitans | |
46.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, wild-type enzyme | Aggregatibacter actinomycetemcomitans | |
76.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme R27K | Aggregatibacter actinomycetemcomitans | |
101.1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme Y187A | Aggregatibacter actinomycetemcomitans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aggregatibacter actinomycetemcomitans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aggregatibacter actinomycetemcomitans |
Storage Stability | Organism |
---|---|
-20°C, buffer comprising 50 mM sodium phosphate, pH 5.8, 50 mm NaCl and 50% glycerol, stable for several months | Aggregatibacter actinomycetemcomitans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl N-acetyl-beta-D-glucosamine + H2O | - |
Aggregatibacter actinomycetemcomitans | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | - |
Aggregatibacter actinomycetemcomitans | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dispersin B | - |
Aggregatibacter actinomycetemcomitans |
DspB | - |
Aggregatibacter actinomycetemcomitans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000083 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme E332Q | Aggregatibacter actinomycetemcomitans | |
0.00033 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme D183N | Aggregatibacter actinomycetemcomitans | |
0.00038 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme R27A | Aggregatibacter actinomycetemcomitans | |
0.00083 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme R27K | Aggregatibacter actinomycetemcomitans | |
0.00122 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme E184Q | Aggregatibacter actinomycetemcomitans | |
0.0015 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme Y278A | Aggregatibacter actinomycetemcomitans | |
0.00417 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme Y278A | Aggregatibacter actinomycetemcomitans | |
0.00717 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme Y187A | Aggregatibacter actinomycetemcomitans | |
0.0235 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, wild-type enzyme | Aggregatibacter actinomycetemcomitans | |
0.06 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, mutant enzyme Y187A | Aggregatibacter actinomycetemcomitans | |
0.927 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, wild-type enzyme | Aggregatibacter actinomycetemcomitans | |
6.08 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | 37°C, pH 5.8, wild-type enzyme | Aggregatibacter actinomycetemcomitans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
wild-type enzyme | Aggregatibacter actinomycetemcomitans |
6.8 | - |
mutant enzyme E184Q | Aggregatibacter actinomycetemcomitans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5: about 65% of maximal activity, pH 7: about 45% of maximal activity, wild-type enzyme | Aggregatibacter actinomycetemcomitans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.147 | - |
- |
Aggregatibacter actinomycetemcomitans | NAG-thiazoline |