Cloned (Comment) | Organism |
---|---|
recombinant His-tagged HexA is produced in the methylotrophic yeast Ogataea minuta (alpha-1,6-mannosyltransferase-deficient). Genes encoding the alpha- and beta-subunits of HexA are integrated into the yeast cell, and the heterodimer is expressed together with its isozymes HexS (alphaalpha) and HexB (betabeta). A total of 57 mg of beta-hexosaminidase isozymes, of which 13 mg was HexA (alphabeta), is produced per liter of medium. The C terminus of the beta-subunit appears to be truncated in Ogataea minuta recombinant HexA | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07686 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme. HexA is purified with immobilized metal affinity column for the His tag attached to the beta-subunit | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
recombinant enzyme, substrate: 4-methylumbelliferyl-6-sulfo-N-acetyl-beta-D-glucosaminide | Homo sapiens |
0.28 | - |
recombinant enzyme, substrate: 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-6-sulfo-N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | ? | - |
? | |
4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-hexosaminidase A | - |
Homo sapiens |