Any feedback?
Literature summary for 3.2.1.52 extracted from
- Characterisation of a secreted N-acetyl-beta-hexosaminidase from Trichinella spiralis (2006), Mol. Biochem. Parasitol., 145, 84-93.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-acetyl-beta-D-galactosamine | - |
Trichinella spiralis |  |
| N-acetyl-beta-D-glucosamine | - |
Trichinella spiralis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.187 | - |
N-acetyl-beta-D-glucosamine | - |
Trichinella spiralis | |
| 0.215 | - |
N-acetyl-beta-D-galactosamine | - |
Trichinella spiralis | |
| 1.732 | - |
N-acetyl-beta-D-glucosamine-6-sulfate | - |
Trichinella spiralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE | Trichinella spiralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichinella spiralis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Trichinella spiralis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no specific activity on N-acetyl-alpha-D-glucosamine and N-acetyl-alpha-D-galactosamine at pH 4.4 and 37°C | Trichinella spiralis |
| 0.00002 | - |
p-nitrophenyl-beta-D-mannose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00007 | - |
p-nitrophenyl-beta-D-glucose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00024 | - |
p-nitrophenyl-alpha-D-xylose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00028 | - |
p-nitrophenyl-beta-D-fucose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00032 | - |
p-nitrophenyl-beta-D-galactose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00033 | - |
p-nitrophenyl-beta-D-xylose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00042 | - |
N,N,N-triacetylchitotriose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00043 | - |
p-nitrophenyl-alpha-D-mannose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00046 | - |
p-nitrophenyl-alpha-D-fucose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00066 | - |
p-nitrophenyl-beta-D-glucuoronide, pH 4.4, 37°C | Trichinella spiralis |
| 0.00079 | - |
N,N-diacetylchitobiose, pH 4.4, 37°C | Trichinella spiralis |
| 0.0018 | - |
4-methylumbelliferyl-N-acetyl-beta-D-glucosamine-6-sulphate, pH 4.4, 37°C | Trichinella spiralis |
| 0.00185 | - |
p-nitrophenyl-alpha-D-glucose, pH 4.4, 37°C | Trichinella spiralis |
| 0.00396 | - |
4-methylumbelliferyl-N-acetyl-beta-D-galactosamine, pH 4.4, 37°C | Trichinella spiralis |
| 0.00832 | - |
4-methylumbelliferyl-N-acetyl-beta-D-glucosamine, pH 4.4, 37°C | Trichinella spiralis |
| 0.01199 | - |
p-nitrophenyl-N-acetyl-beta-D-galactosamine, pH 4.4, 37°C | Trichinella spiralis |
| 0.0126 | - |
2-O(2-acet-aminido-2-deoxy-beta-D-glucopyranosyl)-D-mannose, pH 4.4, 37°C | Trichinella spiralis |
| 0.02908 | - |
p-nitrophenyl-N-acetyl-beta-D-glucosamine, pH 4.4, 37°C | Trichinella spiralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-N-acetyl-beta-D-glucosamine-6-sulfate + H2O | - |
Trichinella spiralis | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine-6-sulfate | - |
? | |
| N,N',N''-triacetylchitotriose + H2O | - |
Trichinella spiralis | ? | - |
? | |
| N,N'-diacetylchitobiose + H2O | - |
Trichinella spiralis | ? | - |
? | |
| p-nitrophenyl-N-acetyl-beta-D-galactosamine + H2O | - |
Trichinella spiralis | N-acetyl-beta-D-galactosamine + p-nitrophenol | - |
? | |
| p-nitrophenyl-N-acetyl-beta-D-glucosamine + H2O | - |
Trichinella spiralis | N-acetyl-beta-D-glucosamine + p-nitrophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-beta-hexosaminidase | - |
Trichinella spiralis |
| N-acetyl-beta-hexosaminidase | - |
Trichinella spiralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 54 | - |
in citrate phosphate buffer with p-nitrophenyl-N-acetyl-beta-D-glucosamine as substrate | Trichinella spiralis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 70 | in citrate phosphate buffer with p-nitrophenyl-N-acetyl-beta-D-glucosamine as substrate | Trichinella spiralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
in citrate phosphate buffer with p-nitrophenyl-N-acetyl-beta-D-glucosamine as substrate | Trichinella spiralis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.4 | 7.4 | in citrate phosphate buffer with p-nitrophenyl-N-acetyl-beta-D-glucosamine as substrate | Trichinella spiralis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.17 | - |
N-acetyl-beta-D-galactosamine | - |
Trichinella spiralis | |
| 15.75 | - |
N-acetyl-beta-D-glucosamine | - |
Trichinella spiralis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
