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Literature summary for 3.2.1.52 extracted from
- Purification and biological characterization of N-acetyl-beta-D-glucosaminidase from Bufo arenarum spermatozoa (2000), Mol. Reprod. Dev., 57, 194-203.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-acetyl-beta-D-galactosamine | competitive | Rhinella arenarum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
p-nitrophenyl-beta-D-N-acetylglucosaminide | - |
Rhinella arenarum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rhinella arenarum | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
1 * 45000, SDS-PAGE | Rhinella arenarum |
| 45000 | 50000 | gel filtration | Rhinella arenarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rhinella arenarum | major sperm glycosidase, plays an important role in toad fertilization | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhinella arenarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhinella arenarum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sperm | - |
Rhinella arenarum | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, stable for several days without appreciable loss of activity | Rhinella arenarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | major sperm glycosidase, plays an important role in toad fertilization | Rhinella arenarum | ? | - |
? | |
| p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O | - |
Rhinella arenarum | p-nitrophenol + beta-D-N-acetylglucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, SDS-PAGE | Rhinella arenarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
hydrolysis of p-nitrophenyl-beta-D-N-acetylglucosaminide | Rhinella arenarum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 50% of maximal activity, 60°C: about 90% of maximal activity, 80°C: about 30% of maximal activity, hydrolysis of p-nitrophenyl-beta-D-N-acetylglucosaminide | Rhinella arenarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
and a small peak at pH 5.0 | Rhinella arenarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6.5 | pH 3.0: 95% of maximal activity, pH 5.0: about 65% of maximal activity, pH 4.5 and pH 6.5: about 45% of maximal activity, pH 7.0: about 25% of maximal activity | Rhinella arenarum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
N-acetyl-beta-D-galactosamine | - |
Rhinella arenarum | |
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