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Literature summary for 3.2.1.52 extracted from

  • Verpoorte, J.A.
    Purification of two beta-N-acetyl-D-glucosaminidases from beef spleen (1972), J. Biol. Chem., 247, 4787-4793.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin
-
Bos taurus

General Stability

General Stability Organism
unstable during chromatography and dialysis Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Bos taurus
Fe2+
-
Bos taurus
Fe3+
-
Bos taurus
Hg2+
-
Bos taurus
p-chloromercuribenzoate
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form B, pH 4.55 Bos taurus
0.15
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form A, pH 4.55 Bos taurus
0.32
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form B, pH 5.5 Bos taurus
0.38
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form A, pH 5.5 Bos taurus
0.85
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form B, pH 4.5 Bos taurus
0.87
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form A, pH 4.55 Bos taurus
1.96
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form A, pH 3.55 Bos taurus
2.38
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form B, pH 3.55 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
Bos taurus
140000
-
sedimentation equilibrium Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Bos taurus
-
N-acetyl-D-hexosamines
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
dithiothreitol 2 mM cause total inhibition Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein neutral sugar Bos taurus
glycoprotein glucosaminylation Bos taurus
glycoprotein sialylation Bos taurus

Purification (Commentary)

Purification (Comment) Organism
A form: 310fold, B form: 260fold, to homogeneity Bos taurus

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.1
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form B Bos taurus
21.1
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form A Bos taurus

Storage Stability

Storage Stability Organism
-20°C, crude extract Bos taurus
2°C, in (NH4)2SO4, stable Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Bos taurus 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
?
N-acetyl-beta-D-hexosaminides + H2O
-
Bos taurus N-acetyl-D-hexosamines
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Bos taurus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Bos taurus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?

Subunits

Subunits Comment Organism
More
-
Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
at pH 4.5, stable for 15 min Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
at 37°C, stable for 15 min Bos taurus