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Literature summary for 3.2.1.52 extracted from
- Purification and characterization of a beta-N-acetylglucosaminidase from Octopus vulgaris. Determination of specificity by using 360-MHz 1H-NMR spectroscopy (1983), Eur. J. Biochem., 132, 469-476.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.527 | - |
phenyl-N-acetyl-beta-D-glucosaminide | - |
Octopus vulgaris |  |
| 0.617 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Octopus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
2 * 27000 + 2 * 34000, SDS-PAGE | Octopus vulgaris |
| 34000 | - |
2 * 27000 + 2 * 34000, SDS-PAGE | Octopus vulgaris |
| 120000 | - |
gel filtration | Octopus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Octopus vulgaris | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Octopus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| types I-IV 150fold, type I to homogeneity | Octopus vulgaris |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Octopus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Octopus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62.9 | - |
substrate: phenyl-N-acetyl-beta-D-glucosaminide | Octopus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | - |
Octopus vulgaris | N-acetyl-D-hexosamines | - |
? | |
| ovalbumin glycopeptides + H2O | ovalbumin glycopeptide AC-A with the sugar composition: Gal,GlcNAc5,Man5,Asn | Octopus vulgaris | ? | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Octopus vulgaris | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Octopus vulgaris | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
| phenyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Octopus vulgaris | phenol + N-acetyl-beta-D-glucosaminide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 27000 + 2 * 34000, SDS-PAGE | Octopus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
forms II and IV | Octopus vulgaris |
| 55 | 65 | form I | Octopus vulgaris |
| 60 | - |
form III | Octopus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
forms I-IV, substrate: phenyl-N-acetyl-beta-D-glucosaminide | Octopus vulgaris |
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