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Literature summary for 3.2.1.52 extracted from

  • Pocsi, I.; Kiss, L.; Nanasi, P.
    Studies on the N-acetyl-beta-D-hexosaminidase B from germinating Lupinus luteus L. seeds: I. Purification and characterization (1990), Biochim. Biophys. Acta, 1039, 110-118.
    View publication on PubMed
Search for more literature for 3.2.1.52

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
 p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside
-
 Lupinus luteus
0.94
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Lupinus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15300
-
 gel filtration, Sephadex G-75 Lupinus luteus
62500
-
 gel filtration, Bio-Gel-60 Lupinus luteus
69000
-
 SDS-PAGE Lupinus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Lupinus luteus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Lupinus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 132.9-176.6fold Lupinus luteus

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Lupinus luteus
a

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Lupinus luteus
-
seed germinating seeds Lupinus luteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lupinus luteus

Storage Stability

Storage Stability Organism
-20°C, stable for several weeks Lupinus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O
-
 Lupinus luteus N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O
-
 Lupinus luteus p-nitrophenol + 2-acetamido-2-deoxy-beta-D-galactopyranose
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Lupinus luteus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Lupinus luteus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 62500-69000, SDS-PAGE Lupinus luteus