Cloned (Comment) | Organism |
---|---|
into Escherichia coli | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
D246N | mutant | Streptomyces sp. |
E314Q | mutant | Streptomyces sp. |
R162H | mutant | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
4-methylumbelliferyl beta-N-acetylglucosaminide | E314Q | Streptomyces sp. | |
0.14 | - |
4-methylumbelliferyl beta-N-acetylglucosaminide | wild type | Streptomyces sp. | |
0.25 | - |
4-methylumbelliferyl beta-N-acetylglucosaminide | D246N | Streptomyces sp. | |
5.67 | - |
4-methylumbelliferyl beta-N-acetylglucosaminide | R162H | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
recombinant SpHex, western blot analysis | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Streptomyces sp. | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
S. plicatus | - |
Purification (Comment) | Organism |
---|---|
SpHex-MBP fusion protein | Streptomyces sp. |
Storage Stability | Organism |
---|---|
4°C, wild type and mutant enzymes, stable for at least 2 weeks | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-N-acetylglucosaminide + H2O | - |
Streptomyces sp. | 4-methylumbelliferone + beta-N-acetylglucosamine | - |
? | |
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Streptomyces sp. | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Streptomyces sp. | N-acetyl-D-hexosamines | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
stable for at least 48 h at room temperature, wild type and mutant enzymes | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
SpHex wild type | Streptomyces sp. |
3.8 | - |
D246N | Streptomyces sp. |
6.5 | - |
R162H | Streptomyces sp. |