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Literature summary for 3.2.1.52 extracted from

  • Mark, B.L.; Wasney, G.A.; Salo, T.J.S.; Khan, A.M.; Cao, Z.; Robbins, P.W.; James, M.N.G.; Triggs-Raine, B.L.
    Structural and functional characterization of Streptomyces plicatus beta-N-acetylhexosaminidase by comparative molecular modeling and site-directed mutagenesis (1998), J. Biol. Chem., 273, 19618-19624.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into Escherichia coli Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
D246N mutant Streptomyces sp.
E314Q mutant Streptomyces sp.
R162H mutant Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
4-methylumbelliferyl beta-N-acetylglucosaminide E314Q Streptomyces sp.
0.14
-
4-methylumbelliferyl beta-N-acetylglucosaminide wild type Streptomyces sp.
0.25
-
4-methylumbelliferyl beta-N-acetylglucosaminide D246N Streptomyces sp.
5.67
-
4-methylumbelliferyl beta-N-acetylglucosaminide R162H Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
recombinant SpHex, western blot analysis Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Streptomyces sp.
-
N-acetyl-D-hexosamines
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
S. plicatus
-

Purification (Commentary)

Purification (Comment) Organism
SpHex-MBP fusion protein Streptomyces sp.

Storage Stability

Storage Stability Organism
4°C, wild type and mutant enzymes, stable for at least 2 weeks Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-N-acetylglucosaminide + H2O
-
Streptomyces sp. 4-methylumbelliferone + beta-N-acetylglucosamine
-
?
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Streptomyces sp. 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
?
N-acetyl-beta-D-hexosaminides + H2O
-
Streptomyces sp. N-acetyl-D-hexosamines
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
stable for at least 48 h at room temperature, wild type and mutant enzymes Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
SpHex wild type Streptomyces sp.
3.8
-
D246N Streptomyces sp.
6.5
-
R162H Streptomyces sp.