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Literature summary for 3.2.1.52 extracted from
- Purification and characterization of an extracellular beta-N-acetylhexosaminidase from Paecilomyces persicinus (1979), J. Bacteriol., 137, 620-626.
General Stability
| General Stability | Organism |
|---|---|
| lyophilization results in marked decrease in activity | Acremonium persicinum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-mercaptoethanol | with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 22%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 20% | Acremonium persicinum |  |
| Cd2+ | with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 41%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 38% | Acremonium persicinum | |
| Hg2+ | with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 24%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 20% | Acremonium persicinum | |
| N-acetyl-D-galactosamine | with 10 mM the hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 26% | Acremonium persicinum | |
| N-acetyl-D-glucosamine | with 10 mM the hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside is reduced to 32% | Acremonium persicinum | |
| Urea | with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 37%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 34% | Acremonium persicinum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.322 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Acremonium persicinum | |
| 0.625 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Acremonium persicinum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Acremonium persicinum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
SDS-PAGE | Acremonium persicinum |
| 100000 | - |
gel filtration | Acremonium persicinum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Acremonium persicinum | - |
N-acetyl-D-hexosamines | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | Acremonium persicinum P-10 | - |
N-acetyl-D-hexosamines | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-mercaptoethanol | with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 22%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 20% | Acremonium persicinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acremonium persicinum | - |
P-10 | - |
| Acremonium persicinum P-10 | - |
P-10 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 68fold | Acremonium persicinum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Acremonium persicinum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.926 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Acremonium persicinum |
| 5.64 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Acremonium persicinum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme, stable | Acremonium persicinum |
| refrigerated temperatures, stable for several days | Acremonium persicinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | - |
Acremonium persicinum | N-acetyl-D-hexosamines | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Acremonium persicinum P-10 | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Acremonium persicinum | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Acremonium persicinum P-10 | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Acremonium persicinum | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Acremonium persicinum P-10 | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 37 | - |
Acremonium persicinum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 70 | - |
Acremonium persicinum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
temperatures above quickly inactivate the enzyme | Acremonium persicinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Acremonium persicinum |
| 5.8 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Acremonium persicinum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 10 | - |
Acremonium persicinum |
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