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Literature summary for 3.2.1.52 extracted from
- Chitinase and beta-N-acetylhexosaminidase from Pycnoporus cinnabarinus (1988), Methods Enzymol., 161, 462-470.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| relatively stable to freezing and thawing in 0.05 M citrate buffer pH 4.5 | Trametes cinnabarina |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | slight inhibition at 10 mM | Trametes cinnabarina |  |
| additional information | not: N-acetyl-D-galactosamine | Trametes cinnabarina | |
| N,N,N,N-tetraacetylchitotetraose | inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside stronger than hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina | |
| N,N,N-triacetylchitotriose | inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside stronger than hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina | |
| N,N-diacetylchitobiose | inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside stronger than hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina | |
| N-acetyl-D-glucosamine | competitive inhibitor | Trametes cinnabarina | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Trametes cinnabarina | |
| 0.54 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Trametes cinnabarina | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trametes cinnabarina | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
SDS-PAGE | Trametes cinnabarina |
| 120000 | - |
gel filtration | Trametes cinnabarina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Trametes cinnabarina | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes cinnabarina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1400fold | Trametes cinnabarina |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Trametes cinnabarina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Trametes cinnabarina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N'-diacetylchitobiose + H2O | - |
Trametes cinnabarina | N-acetyl-D-glucosamine | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Trametes cinnabarina | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Trametes cinnabarina | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Trametes cinnabarina | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
| reduced chitooligosaccharides + H2O | - |
Trametes cinnabarina | N-acetylglucosamine | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | - |
stable at this temperature between pH 2.0-4.0 | Trametes cinnabarina |
| 50 | - |
unstable above | Trametes cinnabarina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
substrate: N,N-diacetylchitobiose and p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina |
| 3.7 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Trametes cinnabarina |
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