Literature summary for 3.2.1.50 extracted from

Birrane, G.; Dassier, A.L.; Romashko, A.; Lundberg, D.; Holmes, K.; Cottle, T.; Norton, A.W.; Zhang, B.; Concino, M.F.; Meiyappan, M.
Structural characterization of the alpha-N-acetylglucosaminidase, a key enzyme in the pathogenesis of Sanfilippo syndrome B (2019), J. Struct. Biol., 205, 65-71 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.01 M nickel chloride, 0.1M Tris (pH 8.5), 1.0 M lithium sulfate	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P54802	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the enzyme has 6 putative N-glycosylation sites at N261, N272, N435, N503, N526 and N532	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
butyl-Sepharose 4 column chromatography and Q-Sepharose column chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HT-1080 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl N-acetyl-alpha-D-glucosaminide + H2O	-	Homo sapiens	4-methylumbelliferone + N-acetyl-alpha-D-glucosamine	-	?

Subunits

Subunits	Comment	Organism
homotrimer	x-ray crystallography	Homo sapiens

Synonyms

Synonyms	Comment	Organism
alpha-acetylglucosaminidase	-	Homo sapiens
NAG	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	alpha-N-acetylglucosaminidase is a key enzyme in the pathogenesis of Sanfilippo syndrome B	Homo sapiens

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Release 2023.1 (January 2023)