Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.50 extracted from

  • Fujita, M.; Tsuchida, A.; Hirata, A.; Kobayashi, N.; Goto, K.; Osumi, K.; Hirose, Y.; Nakayama, J.; Yamanoi, T.; Ashida, H.; Mizuno, M.
    Glycoside hydrolase family 89 alpha-N-acetylglucosaminidase from Clostridium perfringens specifically acts on GlcNAc alpha1,4Gal beta1R at the non-reducing terminus of O-glycans in gastric mucin (2011), J. Biol. Chem., 286, 6479-6489.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.50

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridium perfringens

Protein Variants

Protein Variants Comment Organism
additional information deletion of the C-terminal region containing several carbohydrate-binding module 32 domains significantly reduces the activity for porcine gastric mucin,while activity against GlcNAc1,4-(1-methoxyphenyl)Gal is markedly enhanced. A deletion construct containing the C-terminal carbohydrate-binding module-C2 to carbohydrate-binding module-C6 domains binds strongly to porcine gastric mucin Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal wild-type, pH 7.4, 37°C Clostridium perfringens
0.28
-
 N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal C-terminal deletion mutant, pH 7.4, 37°C Clostridium perfringens
4.3
-
 4-nitrophenyl-N-acetyl-alpha-D-glucosaminide wild-type, pH 7.4, 37°C Clostridium perfringens
7.8
-
 4-nitrophenyl-N-acetyl-alpha-D-glucosaminide C-terminal deletion mutant, pH 7.4, 37°C Clostridium perfringens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 x * 220000, wild-type, x * 120000, C-terminal deletion mutant, SDS-PAGE Clostridium perfringens
220000
-
 x * 220000, wild-type, x * 120000, C-terminal deletion mutant, SDS-PAGE Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens Q8XM24 member of glycosyl hydrolase family 89
-
Clostridium perfringens 13 Q8XM24 member of glycosyl hydrolase family 89
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Clostridium perfringens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
 C-terminal deletion mutant, substrate 4-nitrophenyl-N-acetyl-alpha-D-glucosaminide, pH 7.4, 37°C Clostridium perfringens
0.1
-
 wild-type, substrate 4-nitrophenyl-N-acetyl-alpha-D-glucosaminide, pH 7.4, 37°C Clostridium perfringens
0.2
-
 wild-type, substrate N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal, pH 7.4, 37°C Clostridium perfringens
2.82
-
 C-terminal deletion mutant, substrate N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal, pH 7.4, 37°C Clostridium perfringens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Clostridium perfringens N-acetyl-alpha-D-glucosamine + 4-nitrophenol
-
 ?
4-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Clostridium perfringens 13 N-acetyl-alpha-D-glucosamine + 4-nitrophenol
-
 ?
cell surface mucin + H2O
-
 Clostridium perfringens N-acetyl-alpha-D-glucosamine + ?
-
 ?
cell surface mucin + H2O
-
 Clostridium perfringens 13 N-acetyl-alpha-D-glucosamine + ?
-
 ?
additional information no activity against any of the GlcNAc1,2-(1-methoxyphenyl)Gal, GlcNAc-1,3-(1-methoxyphenyl)Gal, GlcNAc-1,6-(1-methoxyphenyl)Gal, andGlcNAc-1,4-(1-methoxyphenyl)Glc substrates Clostridium perfringens ?
-
 ?
additional information no activity against any of the GlcNAc1,2-(1-methoxyphenyl)Gal, GlcNAc-1,3-(1-methoxyphenyl)Gal, GlcNAc-1,6-(1-methoxyphenyl)Gal, andGlcNAc-1,4-(1-methoxyphenyl)Glc substrates Clostridium perfringens 13 ?
-
 ?
N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal + H2O
-
 Clostridium perfringens N-acetyl-alpha-D-glucosamine + 1-methoxyphenyl-Gal
-
 ?
N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal + H2O
-
 Clostridium perfringens 13 N-acetyl-alpha-D-glucosamine + 1-methoxyphenyl-Gal
-
 ?
porcine gastric mucin + H2O
-
 Clostridium perfringens N-acetyl-alpha-D-glucosamine + ?
-
 ?
porcine gastric mucin + H2O
-
 Clostridium perfringens 13 N-acetyl-alpha-D-glucosamine + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 220000, wild-type, x * 120000, C-terminal deletion mutant, SDS-PAGE Clostridium perfringens

Synonyms

Synonyms Comment Organism
AgnC
-
 Clostridium perfringens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Clostridium perfringens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable up to Clostridium perfringens
50
-
 3 h, phosphate-buffered slaine, 80% residual activity Clostridium perfringens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.69
-
 4-nitrophenyl-N-acetyl-alpha-D-glucosaminide wild-type, pH 7.4, 37°C Clostridium perfringens
0.74
-
 4-nitrophenyl-N-acetyl-alpha-D-glucosaminide C-terminal deletion mutant, pH 7.4, 37°C Clostridium perfringens
1.24
-
 N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal wild-type, pH 7.4, 37°C Clostridium perfringens
6.19
-
 N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal C-terminal deletion mutant, pH 7.4, 37°C Clostridium perfringens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Clostridium perfringens

General Information

General Information Comment Organism
physiological function enzyme acts as specific glycosidase required for degrading alpha-GlcNAc-capped O-glycans of the class III mucin secreted from the stomach and duodenum. The agnC gene-disrupted strain shows significantly reduced growth on the class III mucin-containing medium compared with the wild type strain Clostridium perfringens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.095
-
 4-nitrophenyl-N-acetyl-alpha-D-glucosaminide C-terminal deletion mutant, pH 7.4, 37°C Clostridium perfringens
0.16
-
 4-nitrophenyl-N-acetyl-alpha-D-glucosaminide wild-type, pH 7.4, 37°C Clostridium perfringens
6.2
-
 N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal wild-type, pH 7.4, 37°C Clostridium perfringens
22.1
-
 N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal C-terminal deletion mutant, pH 7.4, 37°C Clostridium perfringens