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Literature summary for 3.2.1.50 extracted from
- Expression and characterization of human recombinant and alpha-N-acetylglucosaminidase (2001), Protein Expr. Purif., 21, 251-259.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the use of secreted enzyme in future enzyme and gene replacement therapy protocols will by severely limited due to its small degree of mannose-6-phosphorylation | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in CHO-K1 cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0166 | - |
O-(alpha-acetamido-2-deoxy-D-glucopyranosyl)-(1-3)-[L-6,3H]idoronic acid | pH 4.5, 37°C, with bovine serum albumin | Homo sapiens |  |
| 1.07 | - |
4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside | pH 4.5, 37°C, without bovine serum albumin | Homo sapiens | |
| 5.34 | - |
4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside | pH 4.5, 37°C, with bovine serum albumin | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
x * 79000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE | Homo sapiens |
| 89000 | - |
x * 89000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern | Homo sapiens |
| phosphoprotein | the specific phosphorylation of recombinant enzyme secreted from transfected CHO cells is significantly lower when compared with a control lysosomal enzyme | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | of cells CHO cells stable transfected | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.057 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside + H2O | - |
Homo sapiens | ? | - |
? | |
| O-(alpha-acetamido-2-deoxy-D-glucopyranosyl)-(1-3)-[L-6,3H]idoronic acid + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 79000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE | Homo sapiens |
| ? | x * 89000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAGLU | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
reaction 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside | Homo sapiens |
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