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Literature summary for 3.2.1.49 extracted from
- The effect of fucoidan from the brown alga Fucus evanescence on the activity of alpha-N-acetylgalactosaminidase of human colon carcinoma cells (2018), Mar. Drugs, 16, E155 .
General Stability
| General Stability | Organism |
|---|---|
| the purified preparation of the enzyme can be stored frozen for a month and retains 100% of its activity after thawing | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaN3 | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.15 | - |
4-nitrophenyl N-acetyl-D-galactosamine | pH 5.2, 37°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55600 | - |
SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P17050 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| DLD-1 cell | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| the purified preparation of the enzyme can be stored frozen for a month and retains 100% of its activity after thawing | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N-acetyl-D-galactosamine + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-galactosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55600, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-NaGalase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 68 | 37°C: about 50% of maximal activity, 68°C: about 50% of maximal activity | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
half-life: 60 min | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0258 | - |
4-nitrophenyl N-acetyl-D-galactosamine | pH 5.2, 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | the enzyme is more than 50% active between pH 4.0 and pH 5.5, at pH 6, the enzyme retains almost 40% of activity | Homo sapiens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | fucoidan reduces the expression of the enzyme in the DLD-1 cells | down |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
4-nitrophenyl N-acetyl-D-galactosamine | pH 5.2, 37°C | Homo sapiens | |
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