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Literature summary for 3.2.1.49 extracted from

  • Cao, H.T.T.; Mikkelsen, M.D.; Lezyk, M.J.; Bui, L.M.; Tran, V.T.T.; Silchenko, A.S.; Kusaykin, M.I.; Pham, T.D.; Truong, B.H.; Holck, J.; Meyer, A.S.
    Novel enzyme actions for sulphated galactofucan dpolymerisation and a new engineering strategy for molecular stabilisation of fucoidan degrading enzymes (2018), Mar. Drugs, 16, 422.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.49

General Stability

General Stability Organism
the purified preparation of the enzyme can be stored frozen for a month and retains 100% of its activity after thawing Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NaN3
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.15
-
 4-nitrophenyl N-acetyl-D-galactosamine pH 5.2, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55600
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P17050
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
DLD-1 cell
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
the purified preparation of the enzyme can be stored frozen for a month and retains 100% of its activity after thawing Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-D-galactosamine + H2O
-
 Homo sapiens 4-nitrophenol + N-acetyl-galactosamine
-
 ?

Subunits

Subunits Comment Organism
? x * 55600, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alpha-NaGalase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 68 37°C: about 50% of maximal activity, 68°C: about 50% of maximal activity Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 half-life: 60 min Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0258
-
 4-nitrophenyl N-acetyl-D-galactosamine pH 5.2, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4 6 the enzyme is more than 50% active between pH 4.0 and pH 5.5, at pH 6, the enzyme retains almost 40% of activity Homo sapiens

Expression

Organism Comment Expression
Homo sapiens fucoidan reduces the expression of the enzyme in the DLD-1 cells down

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.012
-
 4-nitrophenyl N-acetyl-D-galactosamine pH 5.2, 37°C Homo sapiens