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Literature summary for 3.2.1.49 extracted from
- Facile production of Aspergillus niger alpha-N-acetylgalactosaminidase in yeast (2011), Protein Expr. Purif., 81, 106-114.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as inactive enzyme in inclusion bodies, expression in Pichia pastoris strain X33 is not successful, heterologous overexpression in Saccharomyces cerevisiae strain W303-1A without enzyme secretion | Aspergillus niger |
| expression in Saccharomyces cerevisiae | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
2-nitrophenyl-alpha-N-acetylgalactosaminide | pH 3.5, 37°C | Aspergillus niger |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, calculated, x * 76000, SDS-PAGE | Aspergillus niger |
| 54000 | - |
1 * 76000, native enzyme, gel filtration, 1 * 54000, about, sequence calculation, 1 * 70000, recombinant enzyme, SDS-PAGE | Aspergillus niger |
| 70000 | - |
and 130000, gel filtration | Aspergillus niger |
| 70000 | - |
recombinant enzyme, analytical ultracentrifugation and dynamic light scattering, monomeric form | Aspergillus niger |
| 76000 | - |
x * 54000, calculated, x * 76000, SDS-PAGE | Aspergillus niger |
| 76000 | - |
1 * 76000, native enzyme, gel filtration, 1 * 54000, about, sequence calculation, 1 * 70000, recombinant enzyme, SDS-PAGE | Aspergillus niger |
| 130000 | - |
and 70000, gel filtration | Aspergillus niger |
| 130000 | - |
recombinant enzyme, analytical ultracentrifugation and dynamic light scattering, dimeric form | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aspergillus niger | an exoglycosidase specific for the hydrolysis of terminal alpha-linked N-acetylgalactosamine in various sugar chains | ? | - |
? | |
| additional information | Aspergillus niger CCIM K2 | an exoglycosidase specific for the hydrolysis of terminal alpha-linked N-acetylgalactosamine in various sugar chains | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | G5D7B5 | - |
- |
| Aspergillus niger | G5D7B5 | bifunctional alpha-N-acetylgalactosaminidase and alpha-N-acetylgalactosidase | - |
| Aspergillus niger CCIM K2 | G5D7B5 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | sequence contains eight potential N-glycosylation sites. Six asparagines located at residues 14, 52, 58, 88, 320 and 456 are glycosylated | Aspergillus niger |
| glycoprotein | N-glycosylation occurs at six of the eight potential N-glycosylation sites in both wild-type and recombinant enzyme, i.e. residues 14, 52, 58, 88, 320 and 456 | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from cell culture supernatant by three chromatographic steps to homogeneity, recombinant enzyme from Saccharomyces cerevisiae to homogeneity 105.9fold by hydrophobic interaction chromatography, ultrafiltration, cation exchange chromatography, gel filtration, and anion exchange chromatography | Aspergillus niger |
| recombinant protein | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42.3 | - |
pH 3.5, 37°C | Aspergillus niger |
| 42.3 | - |
purified recombinant enzyme, pH 3.5, 37°C | Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM citrate-phosphate buffer within the pH range 2-4, stable for several weeks | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl 2-acetyl-2-deoxy-alpha-D-galactosamine + H2O | - |
Aspergillus niger | 2-nitrophenol + 2-acetyl-2-deoxy-alpha-D-galactosamine | - |
? | |
| 2-nitrophenyl 2-acetyl-2-deoxy-alpha-D-galactosamine + H2O | - |
Aspergillus niger CCIM K2 | 2-nitrophenol + 2-acetyl-2-deoxy-alpha-D-galactosamine | - |
? | |
| 2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O | - |
Aspergillus niger | 2-nitrophenol + alpha-N-acetylgalactosamine | - |
? | |
| 2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O | - |
Aspergillus niger CCIM K2 | 2-nitrophenol + alpha-N-acetylgalactosamine | - |
? | |
| 4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O | - |
Aspergillus niger | 4-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
| 4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O | - |
Aspergillus niger CCIM K2 | 4-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
| additional information | an exoglycosidase specific for the hydrolysis of terminal alpha-linked N-acetylgalactosamine in various sugar chains | Aspergillus niger | ? | - |
? | |
| additional information | the enzyme also exhibits alpha-galactosidase activity | Aspergillus niger | ? | - |
? | |
| additional information | an exoglycosidase specific for the hydrolysis of terminal alpha-linked N-acetylgalactosamine in various sugar chains | Aspergillus niger CCIM K2 | ? | - |
? | |
| additional information | the enzyme also exhibits alpha-galactosidase activity | Aspergillus niger CCIM K2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, calculated, x * 76000, SDS-PAGE | Aspergillus niger |
| dimer | 2 * 70000, recombinant enzyme, SDS-PAGE | Aspergillus niger |
| monomer | 1 * 76000, native enzyme, gel filtration, 1 * 54000, about, sequence calculation, 1 * 70000, recombinant enzyme, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-GalNAc-ase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Aspergillus niger |
| 50 | 55 | recombinant enzyme, substrate 2-nitrophenyl 2-acetyl-2-deoxy-alpha-D-galactosamine | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
48 h, 35% loss of activity | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 4 | recombinant enzyme, substrate 2-nitrophenyl 2-acetyl-2-deoxy-alpha-D-galactosamine | Aspergillus niger |
| 2 | 2.4 | - |
Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | isoelectric focusing | - |
4.4 |
| Aspergillus niger | isoelectric focusing, recombinant enzyme | - |
4.4 |
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