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Literature summary for 3.2.1.49 extracted from
- Induction, purification and characterization of alpha-N-acetylgalactosaminidase from Aspergillus niger (2008), Appl. Microbiol. Biotechnol., 79, 769-774.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 10 mM, 12% residual activity | Aspergillus niger |  |
| additional information | not inhibitory: Ca2+, Mg2+, Mn2+, Ag+ | Aspergillus niger | |
| Pb2+ | 10 mM, 30% residual activity | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.73 | - |
2-nitrophenyl-alpha-N-acetylgalactosaminide | pH 3.0, 35°C | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
6 * 76000, SDS-PAGE | Aspergillus niger |
| 440000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
best producer of alpha-N-acetylgalactosaminase | - |
| Aspergillus niger CCIM K2 | - |
best producer of alpha-N-acetylgalactosaminase | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme may be deglycosylated without loss of activity | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33.5 | - |
pH 3.0, 35°C | Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, about 5% loss of activity | Aspergillus niger |
| 4°C, pH 1.5-4.0, stable for several months | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O | enzyme acts with retention of the anomeric configuration | Aspergillus niger | 2-nitrophenol + alpha-N-acetylgalactosamine | - |
? | |
| 2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O | enzyme acts with retention of the anomeric configuration | Aspergillus niger CCIM K2 | 2-nitrophenol + alpha-N-acetylgalactosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 76000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
stable for at least 3 days | Aspergillus niger |
| 55 | - |
5 h, 50% loss of activity | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
- |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | 4 | - |
Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | isoelectric focusing | - |
4.8 |
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