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Literature summary for 3.2.1.49 extracted from
- Purification and characterization of alpha-N-acetylgalactosaminidase from Clostridium perfringens (2000), IUBMB Life, 50, 91-97.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential application in the field of erythrocyte conversion technology | Clostridium perfringens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.35 | - |
p-nitrophenyl-N-acetyl-alpha-D-galactosaminide | pH 7.0, 37°C | Clostridium perfringens |  |
| 1.58 | - |
o-nitrophenyl-N-acetyl-alpha-D-galactosaminide | pH 7.0, 37°C | Clostridium perfringens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72100 | - |
x * 72100 Da, SDS-PAGE | Clostridium perfringens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium perfringens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 43.92 | - |
- |
Clostridium perfringens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 12 months | Clostridium perfringens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is highly selective for terminal N-acetylgalactosamine residues, sugars other than N-acetyl-alpha-D-galactosaminide are not substrates. the enzyme degrades blood A epitope | Clostridium perfringens | ? | - |
? | |
| o-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O | - |
Clostridium perfringens | o-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
| p-nitrophenyl-N-acetyl-alpha-D-galactosaminide + H2O | - |
Clostridium perfringens | p-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72100 Da, SDS-PAGE | Clostridium perfringens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Clostridium perfringens |
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