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Literature summary for 3.2.1.49 extracted from

  • Zhu, A.; Monahan c.; Wang Z.; Goldstein J.
    Expression, purification and characterization of recombinant alpha-N-acetylgalactosaminidase produced in the yeast Pichia pastoris (1996), Protein Expr. Purif., 8, 456-462.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in yeast Pichia pastoris Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
p-nitrophenyl-alpha-N-acetylgalactosaminide
-
Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
43000, SDS-PAGE Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
chicken
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51.2
-
recombinant enzyme Gallus gallus
56.4
-
native enzyme Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-alpha-N-acetylgalactosaminide + H2O
-
Gallus gallus p-nitrophenol + alpha-N-acetylgalactosamine
-
?

Subunits

Subunits Comment Organism
monomer 43000, SDS-PAGE Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7
-
-
Gallus gallus