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Literature summary for 3.2.1.48 extracted from

  • Comfort, D.A.; Chou, C.J.; Conners, S.B.; VanFossen, A.L.; Kelly, R.M.
    Functional-genomics-based identification and characterization of open reading frames encoding alpha-glucoside-processing enzymes in the hyperthermophilic archaeon Pyrococcus furiosus (2008), Appl. Environ. Microbiol., 74, 1281-1283.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.051
-
4-nitrophenyl alpha-D-glucopyranoside 90°C, pH not specified in the publication Pyrococcus furiosus
3.2
-
panose 90°C, pH not specified in the publication Pyrococcus furiosus
5
-
isomaltose 90°C, pH not specified in the publication Pyrococcus furiosus
7.1
-
maltose 90°C, pH not specified in the publication Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pyrococcus furiosus pullulan is likely degraded extracellularly by an amylopullulanase and further hydrolyzed by the PF0132 protein after intracellular transport ?
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U4F6
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Pyrococcus furiosus 4-nitrophenol + alpha-D-glucopyranose
-
?
isomaltose + H2O
-
Pyrococcus furiosus ?
-
?
maltose + H2O comparable catalytic efficiencies for panose and maltose Pyrococcus furiosus ?
-
?
additional information pullulan is likely degraded extracellularly by an amylopullulanase and further hydrolyzed by the PF0132 protein after intracellular transport Pyrococcus furiosus ?
-
?
panose + H2O comparable catalytic efficiencies for panose and maltose Pyrococcus furiosus ?
-
?

Synonyms

Synonyms Comment Organism
isomaltase
-
Pyrococcus furiosus
PF0132
-
Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.9
-
4-nitrophenyl alpha-D-glucopyranoside 90°C, pH not specified in the publication Pyrococcus furiosus
58.8
-
isomaltose 90°C, pH not specified in the publication Pyrococcus furiosus
278
-
panose 90°C, pH not specified in the publication Pyrococcus furiosus
1600
-
maltose 90°C, pH not specified in the publication Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function involved in processing alpha-1,6-glucans. Pullulan is likely degraded extracellularly by an amylopullulanase and further hydrolyzed by the PF0132 protein after intracellular transport Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.8
-
isomaltose 90°C, pH not specified in the publication Pyrococcus furiosus
85.6
-
panose 90°C, pH not specified in the publication Pyrococcus furiosus
225
-
maltose 90°C, pH not specified in the publication Pyrococcus furiosus
429
-
4-nitrophenyl alpha-D-glucopyranoside 90°C, pH not specified in the publication Pyrococcus furiosus