KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
4-nitrophenyl alpha-D-glucopyranoside | 90°C, pH not specified in the publication | Pyrococcus furiosus | |
3.2 | - |
panose | 90°C, pH not specified in the publication | Pyrococcus furiosus | |
5 | - |
isomaltose | 90°C, pH not specified in the publication | Pyrococcus furiosus | |
7.1 | - |
maltose | 90°C, pH not specified in the publication | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pyrococcus furiosus | pullulan is likely degraded extracellularly by an amylopullulanase and further hydrolyzed by the PF0132 protein after intracellular transport | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U4F6 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
isomaltose + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
maltose + H2O | comparable catalytic efficiencies for panose and maltose | Pyrococcus furiosus | ? | - |
? | |
additional information | pullulan is likely degraded extracellularly by an amylopullulanase and further hydrolyzed by the PF0132 protein after intracellular transport | Pyrococcus furiosus | ? | - |
? | |
panose + H2O | comparable catalytic efficiencies for panose and maltose | Pyrococcus furiosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
isomaltase | - |
Pyrococcus furiosus |
PF0132 | - |
Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.9 | - |
4-nitrophenyl alpha-D-glucopyranoside | 90°C, pH not specified in the publication | Pyrococcus furiosus | |
58.8 | - |
isomaltose | 90°C, pH not specified in the publication | Pyrococcus furiosus | |
278 | - |
panose | 90°C, pH not specified in the publication | Pyrococcus furiosus | |
1600 | - |
maltose | 90°C, pH not specified in the publication | Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
physiological function | involved in processing alpha-1,6-glucans. Pullulan is likely degraded extracellularly by an amylopullulanase and further hydrolyzed by the PF0132 protein after intracellular transport | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.8 | - |
isomaltose | 90°C, pH not specified in the publication | Pyrococcus furiosus | |
85.6 | - |
panose | 90°C, pH not specified in the publication | Pyrococcus furiosus | |
225 | - |
maltose | 90°C, pH not specified in the publication | Pyrococcus furiosus | |
429 | - |
4-nitrophenyl alpha-D-glucopyranoside | 90°C, pH not specified in the publication | Pyrococcus furiosus |