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Literature summary for 3.2.1.48 extracted from

  • Minai-Tehrani, D.; Fooladi, N.; Minoui, S.; Sobhani-Damavandifar, Z.; Aavani, T.; Heydarzadeh, S.; Attar, F.; Ghaffari, M.; Nazem, H.
    Structural changes and inhibition of sucrase after binding of scopolamine (2010), Eur. J. Pharmacol., 635, 23-26.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ranitidine noncompetitive. Ranitidine can bind to both free enzyme and enzyme-substrate complex, which is accompanied by reduction of emission intensity and red shift of fluorescence spectra Saccharomyces cerevisiae
Scopolamine i.e. hyoscine, is commonly used as an anticholinergic drug to relieve nausea, vomiting and dizziness of amotion sickness as well as recovery from anesthesia and surgery. It shows non-competitive inhibition at different concentrations of 0.6-3.6 mM Homo sapiens
Scopolamine
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, overview Homo sapiens
11.5
-
sucrose pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + H2O Homo sapiens
-
alpha-D-glucose + D-fructose
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
Homo sapiens alpha-D-glucose + D-fructose
-
?
sucrose + H2O
-
Saccharomyces cerevisiae alpha-D-glucose + D-fructose
-
?
sucrose + H2O the absolute enzyme exhibits a flat negative trough, indicating the presence of alpha-helices and beta-sheet structures in the enzyme Homo sapiens alpha-D-glucose + D-fructose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 8 assay at, 20% less activity at pH 8.0 compared to pH 7.0 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
Scopolamine pH 8.0, 37°C Homo sapiens
2.3
-
ranitidine pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.2
-
pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae ranitidine