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Literature summary for 3.2.1.48 extracted from
- Identification and enzymatic characterization of the maltose-inducible alpha-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis (1998), J. Bacteriol., 180, 2574-2578.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| maltose | - |
Bacillus subtilis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, the gene coding the enzyme has been identified in the ORF yvdL/malL | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.135 | - |
maltose | - |
Bacillus subtilis | |
| 0.21 | - |
p-nitrophenyl-alpha-D-glucoside | - |
Bacillus subtilis | |
| 0.455 | - |
isomaltose | - |
Bacillus subtilis | |
| 10.2 | - |
sucrose | - |
Bacillus subtilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + H2O | Bacillus subtilis | - |
2 glucose | - |
? | |
| sucrose + H2O | Bacillus subtilis | - |
D-glucose + D-fructose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
maltose-inducible alpha-glucosidase MalL, sucrase-isomaltase-maltase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| produced and purified in Escherichia coli RB791/pMalL | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.47 | - |
maltose | Bacillus subtilis |
| 2.02 | - |
isomaltose | Bacillus subtilis |
| 4.47 | - |
sucrose | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltose + H2O | - |
Bacillus subtilis | 2 D-glucose | - |
? | |
| maltose + H2O | - |
Bacillus subtilis | 2 glucose | - |
? | |
| maltose + H2O | - |
Bacillus subtilis | 2 D-glucose | - |
? | |
| p-nitrophenyl-alpha-glucoside + H2O | - |
Bacillus subtilis | p-nitrophenol + D-glucose | - |
? | |
| sucrose + H2O | - |
Bacillus subtilis | D-glucose + D-fructose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
with PNPG as substrate, at pH 5.75 and 8.0 activity reduced to 50% | Bacillus subtilis |
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