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Literature summary for 3.2.1.46 extracted from

  • Sakai, N.; Inui, K.; Midorikawa, M.; Okuno, Y.; Ueda, S.; Iwamatsu, A.; Okada, S.
    Purification and characterization of galactocerebrosidase from human lymphocytes (1994), J. Biochem., 116, 615-620.
    View publication on PubMed
Search for more literature for 3.2.1.46

General Stability

General Stability Organism
bovine serum albumin for stabilization Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 galactocerebroside taurocholate system Homo sapiens
0.017
-
 6-hexadecanoylamino-4-methylbelliferyl-beta-D-galactopyranoside cholate system Homo sapiens
0.025
-
 galactocerebroside cholate system Homo sapiens
0.045
-
 6-hexadecanoylamino-4-methylbelliferyl-beta-D-galactopyranoside taurocholate system Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration, SDS-PAGE, N-terminal sequencing Homo sapiens
50000
-
 urine, gel filtration, SDS-PAGE, N-terminal sequencing Homo sapiens
70000
-
 gel filtration, SDS-PAGE, N-terminal sequencing Homo sapiens
77000
-
 amino acid analysis Homo sapiens
90000
-
 gel filtration, SDS-PAGE, N-terminal sequencing Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactosyl-N-acylsphingosine + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 mutant enzyme /Krabbe disease
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
 Homo sapiens
-
skin fibroblast in culture Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-hexadecanoylamino-4-methylumbelliferyl-beta-D-galactopyranoside + H2O
-
 Homo sapiens 6-hexadecanoylamino-4-methylumbelliferone + beta-D-galactopyranose
-
 ?
D-galactosyl-N-acylsphingosine + H2O
-
 Homo sapiens D-galactose + N-acylsphingosine
-
 ?
D-galactosyl-N-acylsphingosine + H2O
-
 Homo sapiens ?
-
 ?
galactocerebroside + H2O
-
 Homo sapiens D-galactose + N-acylceramide
-
 ?
galactosylceramide + H2O
-
 Homo sapiens D-galactose + N-acylceramide
-
 ?
GM1 ganglioside + H2O
-
 Homo sapiens D-galactose + N-acylceramide
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 taurocholate activated Homo sapiens
4.4
-
 6-hexadecanoylamino-4-methylbelliferyl-beta-D-galactopyranoside, cholate system Homo sapiens
4.6
-
 galactocerebroside, cholate system Homo sapiens
5.2
-
 6-hexadecanoylamino-4-methylbelliferyl-beta-D-galactopyranoside, cholate system Homo sapiens