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Literature summary for 3.2.1.45 extracted from

  • Steet, R.A.; Chung, S.; Wustman, B.; Powe, A.; Do, H.; Kornfeld, S.A.
    The iminosugar isofagomine increases the activity of N370S mutant acid beta-glucosidase in Gaucher fibroblasts by several mechanisms (2006), Proc. Natl. Acad. Sci. USA, 103, 13813-13818.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.45

Activating Compound

Activating Compound Comment Organism Structure
isofagomine GlcCerase activity is enhanced in normal fibroblasts 1.3fold after treatment with 0.03 mM isofagomine for 5 days Homo sapiens

Protein Variants

Protein Variants Comment Organism
N370S decreased activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
isofagomine
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
CCD-43Sk cell
-
 Homo sapiens
-
fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens methylumbelliferone + beta-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
GlcCerase
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000005
-
 wild type enzyme, at pH 7.2 Homo sapiens isofagomine
0.000009
-
 wild type enzyme, at pH 6.4 Homo sapiens isofagomine
0.000018
-
 mutant enzyme N370S, at pH 5.2 Homo sapiens isofagomine
0.000026
-
 mutant enzyme N370S, at pH 6.4 Homo sapiens isofagomine
0.00003
-
 wild type enzyme, at pH 5.2 Homo sapiens isofagomine
0.000128
-
 mutant enzyme N370S, at pH 7.2 Homo sapiens isofagomine