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Literature summary for 3.2.1.45 extracted from
- Isolation and characterization of glucocerebrosidase from human placental tissue (1973), J. Biol. Chem., 248, 5256-5261.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| heat-stable factor | - |
Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes in later stages of chromatographic separation | Homo sapiens |
| glycerol stabilizes in later stages of chromatographic separation | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
beta-D-glucocerebroside | - |
Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glucocerebroside + H2O | Homo sapiens | - |
glucose + ceramide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.6 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Homo sapiens | methylumbelliferone + glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Homo sapiens | 4-nitrophenol + D-glucopyranose | - |
? | |
| glucocerebroside + H2O | - |
Homo sapiens | glucose + ceramide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 60000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
addition of heat-stable factor shifts pH-optimum for hydrolysis of methylumbelliferyl-beta-D-glucose from 6.5 to 5.0 | Homo sapiens |
| 6 | 6.6 | - |
Homo sapiens |
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