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Literature summary for 3.2.1.41 extracted from

  • Habib-ur-Rehma, H.; Siddiqui, M.; Qayyum, A.; Bano, A.; Rashid, N.
    Gene expression in Escherichia coli and purification of recombinant type II pullulanase from a hyperthermophilic archaeon, Pyrobaculum calidifontis (2018), Pak. J. Zool., 50, 1381-1386 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrobaculum calidifontis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ca2+-independent enzyme Pyrobaculum calidifontis

Organism

Organism UniProt Comment Textmining
Pyrobaculum calidifontis A3MWL6
-
-
Pyrobaculum calidifontis VA1 A3MWL6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrobaculum calidifontis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Pyrobaculum calidifontis maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
?
amylopectin + H2O
-
Pyrobaculum calidifontis VA1 maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
?
pullulan + H2O
-
Pyrobaculum calidifontis maltotriose + maltose
-
?
pullulan + H2O
-
Pyrobaculum calidifontis VA1 maltotriose + maltose
-
?
starch + H2O
-
Pyrobaculum calidifontis maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
?
starch + H2O
-
Pyrobaculum calidifontis VA1 maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
?

Synonyms

Synonyms Comment Organism
Pcal-1616
-
Pyrobaculum calidifontis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Pyrobaculum calidifontis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 110 60°C: about 60% of maximal activity, 110°C: about 90% of maximal activity Pyrobaculum calidifontis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Pyrobaculum calidifontis