Literature summary for 3.2.1.41 extracted from

Kahar, U.M.; Chan, K.G.; Sani, M.H.; Mohd Noh, N.I.; Goh, K.M.
Effects of single and co-immobilization on the product specificity of type I pullulanase from Anoxybacillus sp. SK3-4 (2017), Int. J. Biol. Macromol., 104, 322-332 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21(DE3)	Anoxybacillus ayderensis

Organism

Organism	UniProt	Comment	Textmining
Anoxybacillus ayderensis	EPZ37738	-	-
Anoxybacillus ayderensis DSM 28779	EPZ37738	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Anoxybacillus ayderensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pullulan + H2O	soluble enzyme is highly reactive towards pullulan but was unable to degrade short branches in starch	Anoxybacillus ayderensis	maltotriose + ?	-	?
pullulan + H2O	soluble enzyme is highly reactive towards pullulan but was unable to degrade short branches in starch	Anoxybacillus ayderensis DSM 28779	maltotriose + ?	-	?
starch + H2O	following immobilization through covalent attachment to three epoxides (ReliZyme EP403/M, ImmobeadIB-150P, and Immobead IB-150A) and an amino-epoxide (ReliZyme HFA403/M), all PulASK derivatives are active on both short and long branches in starch producing reducing sugars (predominantly maltotriose) and oligosaccharides (chain lenght G8 and higher), respectively, a feature that is absent in the free enzyme. Individual or coimmobilization causes changes in the product specificity, presumably due to changes in the enzyme binding pocket caused by the influence of carrier surface properties (hydrophobicor hydrophilic) and the lengths of the spacer arms	Anoxybacillus ayderensis	maltotriose + oligosaccharides	-	?
starch + H2O	following immobilization through covalent attachment to three epoxides (ReliZyme EP403/M, ImmobeadIB-150P, and Immobead IB-150A) and an amino-epoxide (ReliZyme HFA403/M), all PulASK derivatives are active on both short and long branches in starch producing reducing sugars (predominantly maltotriose) and oligosaccharides (chain lenght G8 and higher), respectively, a feature that is absent in the free enzyme. Individual or coimmobilization causes changes in the product specificity, presumably due to changes in the enzyme binding pocket caused by the influence of carrier surface properties (hydrophobicor hydrophilic) and the lengths of the spacer arms	Anoxybacillus ayderensis DSM 28779	maltotriose + oligosaccharides	-	?

Synonyms

Synonyms	Comment	Organism
PulASK	-	Anoxybacillus ayderensis

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Release 2023.1 (January 2023)