Literature summary for 3.2.1.41 extracted from

Li, J.X.; Wang, S.Q.; Du, Q.S.; Wei, H.; Li, X.M.; Meng, J.Z.; Wang, Q.Y.; Xie, N.Z.; Huang, R.B.; Chou, K.C.
Simulated protein thermal detection (SPTD) for enzyme thermostability study and an application example for pullulanase from Bacillus deramificans (2018), Curr. Pharm. Des., 24, 4023-4033 .

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Protein Variants

Protein Variants	Comment	Organism
D332H/D398Y	mutant enzyme shows remarkable improvement of thermal stability in higher temperature range (above 55 °C). The best temperature of the relative activity moves to 60°C. The activity performance in the middle temperature range (40 to 55°C) is worse than that of the wild type pullulanase	Bacillus deramificans
D332H/D398Y/V390N	the activity performance of mutations D332H/D398Y/V390N is better than that of mutations D332H/D398Y/V390S in all temperature range from 40°C to 65°C	Bacillus deramificans
D332H/D398Y/V390S	the activity performance of the mutant enzyme is better than the wild pullulanase-BDPulA in all temperature range from 40°C to 65°C. In the temperature range lower than 55°C the activity is worse than mutation V390S alone, and in the temperature range higher than 55°C the activity of the mutant enzyme is worse than the mutation D332H/D398Y, but better than the wild type enzyme and V390S-mutated BDPulA	Bacillus deramificans

Organism

Organism	UniProt	Comment	Textmining
Bacillus deramificans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bacillus deramificans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pullulan + H2O	-	Bacillus deramificans	?	-	?

Synonyms

Synonyms	Comment	Organism
BDPulA324	-	Bacillus deramificans
CAC60157	locus name	Bacillus deramificans

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Release 2023.1 (January 2023)