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Literature summary for 3.2.1.41 extracted from
- The N-terminal domain of the pullulanase from Anoxybacillus sp. WB42 modulates enzyme specificity and thermostability (2018), ChemBioChem, 19, 949-955 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A90D | mutant enzyme shows increase in thermostability as compared to wild-type enzyme, increase in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| A90G | mutant enzyme shows increase in thermostability as compared to wild-type enzyme, increase in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| A90P | mutant enzyme shows increase in thermostability as compared to wild-type enzyme, increase in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| A90S | mutant enzyme shows increase in thermostability as compared to wild-type enzyme, increase in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| H5A | mutant enzyme shows the same thermostability as wild-type enzyme. No activity with pullulan | Anoxybacillus sp. WB42 |
| H5A/R6A/T7A | mutant enzyme shows increase in thermostability as compared to wild-type enzyme, decrease in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| L173D | mutant enzyme shows increase in thermostability as compared to wild-type enzyme, increase in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| M88D | mutant enzyme shows increase in thermostability as compared to wild-type enzyme. No activity with pullulan | Anoxybacillus sp. WB42 |
| Q87A | mutant enzyme shows increase in thermostability as compared to wild-type enzyme, decrease in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| Q87A/L173D | mutant enzyme shows increase in thermostability as compared to wild-type enzyme, decrease in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| Q87G | mutant enzyme shows slight decrease in thermostability as compared to wild-type enzyme. No activity with pullulan | Anoxybacillus sp. WB42 |
| R6A | mutant enzyme shows the same thermostability as wild-type enzyme. No activity with pullulan | Anoxybacillus sp. WB42 |
| R93A | mutant enzyme shows slight decrease in thermostability as compared to wild-type enzyme, slight decrease in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| R93E | mutant enzyme shows slight decrease in thermostability as compared to wild-type enzyme, decrease in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| R93K | mutant enzyme shows slight increase in thermostability as compared to wild-type enzyme, decrease in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| R93T | mutant enzyme shows slight increase in thermostability as compared to wild-type enzyme, slight decrease in kcat/Km for pullulan | Anoxybacillus sp. WB42 |
| T7A | mutant enzyme shows the same thermostability as wild-type enzyme. No activity with pullulan | Anoxybacillus sp. WB42 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
pullulan | Km-value: 16.5 mg/ml, pH 5.5, 60°C, mutant enzyme H5A/R6A/T7A | Anoxybacillus sp. WB42 |  |
| additional information | - |
pullulan | Km-value: 20.1 mg/ml, pH 5.5, 60°C, mutant enzyme R93T | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 22.3 mg/ml, pH 5.5, 60°C, mutant enzyme R93A | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 22.5 mg/ml, pH 5.5, 60°C, wild-type enzyme | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 24.4 mg/ml, pH 5.5, 60°C, mutant enzyme A90G | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 24.6 mg/ml, pH 5.5, 60°C, mutant enzyme A90D | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 29.7 mg/ml, pH 5.5, 60°C, mutant enzyme A90P | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 31.2 mg/ml, pH 5.5, 60°C, mutant enzyme Q87A/L173D | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 4.7 mg/ml, pH 5.5, 60°C, mutant enzyme R93K | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 45.0 mg/ml, pH 5.5, 60°C, mutant enzyme A90S | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 5.6 mg/ml, pH 5.5, 60°C, mutant enzyme R93E | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 5.8 mg/ml, pH 5.5, 60°C, mutant enzyme L173D | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 6.0 mg/ml, pH 5.5, 60°C, mutant enzyme DELTA(N) | Anoxybacillus sp. WB42 | |
| additional information | - |
pullulan | Km-value: 6.8 mg/ml, pH 5.5, 60°C, mutant enzyme Q87A | Anoxybacillus sp. WB42 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anoxybacillus sp. WB42 | A0A286QJY9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Anoxybacillus sp. WB42 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + H2O | - |
Anoxybacillus sp. WB42 | ? | - |
? | |
| pullulan + H2O | - |
Anoxybacillus sp. WB42 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amylopullulanase | - |
Anoxybacillus sp. WB42 |
| PulWB42 | - |
Anoxybacillus sp. WB42 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Anoxybacillus sp. WB42 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65.8 | - |
T50, mutant enzyme Q87G | Anoxybacillus sp. WB42 |
| 66.4 | - |
T50, mutant enzyme R93A, mutant enzyme R93E, mutant enzyme A90G | Anoxybacillus sp. WB42 |
| 66.6 | - |
T50, wild-type enzyme, mutant enzyme H5A, mutant enzyme R6A | Anoxybacillus sp. WB42 |
| 66.8 | - |
T50, mutant enzyme R93K, mutant enzyme R93T, mutant enzyme R93T | Anoxybacillus sp. WB42 |
| 67 | - |
T50, mutant enzyme A90P, mutant enzyme A90D | Anoxybacillus sp. WB42 |
| 68.2 | - |
T50, mutant enzyme H5A/R6A/T7A | Anoxybacillus sp. WB42 |
| 68.4 | - |
T50, mutant enzyme M88D | Anoxybacillus sp. WB42 |
| 69 | - |
T50, mutant enzyme Q87A, T50, mutant enzyme L173D, mutant enzyme Q87A/L173D | Anoxybacillus sp. WB42 |
| 69.4 | - |
T50, mutant enzyme A90S | Anoxybacillus sp. WB42 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 44.7 | - |
pullulan | pH 5.5, 60°C, mutant enzyme DELTA(N) | Anoxybacillus sp. WB42 | |
| 103.3 | - |
pullulan | pH 5.5, 60°C, mutant enzyme Q87A | Anoxybacillus sp. WB42 | |
| 128.4 | - |
pullulan | pH 5.5, 60°C, wild-type enzyme | Anoxybacillus sp. WB42 | |
| 182.2 | - |
pullulan | pH 5.5, 60°C, mutant enzyme L173D | Anoxybacillus sp. WB42 | |
| 205.6 | - |
pullulan | pH 5.5, 60°C, mutant enzyme R93E | Anoxybacillus sp. WB42 | |
| 244.4 | - |
pullulan | pH 5.5, 60°C, mutant enzyme R93K | Anoxybacillus sp. WB42 | |
| 285.5 | - |
pullulan | pH 5.5, 60°C, mutant enzyme A90D | Anoxybacillus sp. WB42 | |
| 306.2 | - |
pullulan | pH 5.5, 60°C, mutant enzyme A90P | Anoxybacillus sp. WB42 | |
| 327.2 | - |
pullulan | pH 5.5, 60°C, mutant enzyme A90G | Anoxybacillus sp. WB42 | |
| 341 | - |
pullulan | pH 5.5, 60°C, mutant enzyme A90S | Anoxybacillus sp. WB42 | |
| 342.1 | - |
pullulan | pH 5.5, 60°C, mutant enzyme H5A/R6A/T7A | Anoxybacillus sp. WB42 | |
| 357.5 | - |
pullulan | pH 5.5, 60°C, mutant enzyme Q87A/L173D | Anoxybacillus sp. WB42 | |
| 417.4 | - |
pullulan | pH 5.5, 60°C, mutant enzyme R93A | Anoxybacillus sp. WB42 | |
| 492.4 | - |
pullulan | pH 5.5, 60°C, mutant enzyme R93T | Anoxybacillus sp. WB42 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Anoxybacillus sp. WB42 |
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