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Literature summary for 3.2.1.41 extracted from
- Magnesium ions increase the activity of Bacillus deramificans pullulanase expressed by Brevibacillus choshinensis (2016), Appl. Microbiol. Biotechnol., 100, 7115-7123 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Brevibacillus choshinensis | Bacillus deramificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
pullulan | Km: 1.01 mg/ml, pH 4.5, 60°C, 60 mM Mg2+ | Bacillus deramificans |  |
| additional information | - |
pullulan | Km: 1.06 mg/ml, pH 4.5, 60°C, without Mg2+ | Bacillus deramificans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus deramificans | - |
synthetic construct | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pullulan + H2O | - |
Bacillus deramificans | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Bacillus deramificans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 499 | - |
pullulan | pH 4.5, 60°C, without Mg2+ | Bacillus deramificans | |
| 3000 | - |
pullulan | pH 4.5, 60°C, 60 mM Mg2+ | Bacillus deramificans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
assay at | Bacillus deramificans |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bacillus deramificans | magnesium ion addition represses transcription of cell wall protein (HWP). Because the pullulanase gene and HWP have identical promoters, pullulanase gene transcription is also inhibited. When cultured in medium without added magnesium ions, Brevibacillus choshinensis mainly produced a thermolabile, inactive form of pullulanase. The addition of magnesium ions leads to the production of a thermostable, active form of pullulanase. When pullulanase is expressed slowly, it tends to fold into an active form | down |
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