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Literature summary for 3.2.1.41 extracted from
- Cloning, overexpression and characterization of a thermostable pullulanase from Thermus thermophilus HB27 (2014), Protein Expr. Purif., 95, 22-27 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, enhances activity 1.4fold | Thermus thermophilus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the enzyme can be employed for industrial saccharification of starch | Thermus thermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli using pHsh and pET28a vectors. The pHsh expression system produces a 3.6fold higher recombinant pullulanase than pET28a | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cr3+ | 5 mM, 34% inhibition, pH 6.5, 70°C | Thermus thermophilus | |
| Cu2+ | 5 mM, complete inhibition, pH 6.5, 70°C | Thermus thermophilus | |
| Hg2+ | 5 mM, complete inhibition, pH 6.5, 70°C | Thermus thermophilus | |
| Ni2+ | 5 mM, 31% inhibition, pH 6.5, 70°C | Thermus thermophilus | |
| Pb2+ | 5 mM, 75% inhibition, pH 6.5, 70°C | Thermus thermophilus | |
| SDS | 10 mM, 96% inhibition, pH 6.5, 70°C | Thermus thermophilus | |
| Zn2+ | 5 mM, 58% inhibition, pH 6.5, 70°C | Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
pullulan | Km-value: 0.0031 mg/ml | Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 5 mM, enhances activity 1.7fold, pH 6.5, 70°C | Thermus thermophilus | |
| Mg2+ | 5 mM, enhances activity 1.2fold, pH 6.5, 70°C | Thermus thermophilus | |
| Mn2+ | 5 mM, enhances activity up to 3fold, pH 6.5, 70°C | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
SDS-PAGE | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q72ID4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pullulan + H2O | this enzyme can attack alpha-1,6- and alpha-1,4-glycosidic linkages in pullulan, and produce a mixture of maltotriose, maltose and glucose | Thermus thermophilus | maltotriose + maltose + D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TTC1198 | - |
Thermus thermophilus |
| TTHpu | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
His6-tagged recombinant enzyme, at pH 6.5 | Thermus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 95 | 45°C: about 60% of maximal activity, 95°C: about 55% of maximal activity | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
1 h, residual activity is more than 90% | Thermus thermophilus |
| 70 | - |
1 h, residual activity is more than 90%. More than 70% of the activity is retained after 1 h at pH 6-8 with the absence of the substrate | Thermus thermophilus |
| 80 | - |
half-life: 2 h | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH 5.0: about 65% of maximal activity, pH 8.5: about 65% of maximal activity | Thermus thermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 1 h, 70°C, stable | Thermus thermophilus |
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