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Literature summary for 3.2.1.41 extracted from
- Sequence analysis and biochemical properties of an acidophilic and hyperthermophilic amylopullulanase from Thermofilum pendens (2018), Int. J. Biol. Macromol., 114, 235-243 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologously expressed in Escherichia coli | Thermofilum pendens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
pullulan | Km-value: 0.08 mg/ml, pH 3.5, 95°C | Thermofilum pendens |  |
| additional information | - |
amylose | Km-value: 0.71 mg/ml, pH 3.5, 95°C | Thermofilum pendens | |
| additional information | - |
amylopectin | Km-value: 3.95 mg/ml, pH 3.5, 95°C | Thermofilum pendens | |
| additional information | - |
starch | Km-value: 7.29 mg/ml, pH 3.5, 95°C | Thermofilum pendens | |
| 0.25 | - |
alpha-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
| 0.71 | - |
gamma-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
| 5.16 | - |
beta-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65500 | - |
SDS-PAGE | Thermofilum pendens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermofilum pendens | A1RWP0 | - |
- |
| Thermofilum pendens DSM 2475 | A1RWP0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermofilum pendens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Thermofilum pendens | ? | - |
? | |
| amylopectin + H2O | the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C | Thermofilum pendens | ? | - |
? | |
| amylopectin + H2O | the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C | Thermofilum pendens DSM 2475 | ? | - |
? | |
| amylose + H2O | - |
Thermofilum pendens | ? | - |
? | |
| amylose + H2O | - |
Thermofilum pendens DSM 2475 | ? | - |
? | |
| beta-cyclodextrin + H2O | in the initial stages, the ring-opening reactions of 6-O-glucosyl-beta-cyclodextrin, 6-O-maltosyl-beta-cyclodextrin and the debranching reactions of 6-O-maltooctaosyl-beta-cyclodextrin are firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced | Thermofilum pendens | ? | - |
? | |
| beta-cyclodextrin + H2O | in the initial stages, the ring-opening reactions of 6-O-glucosyl-beta-cyclodextrin, 6-O-maltosyl-beta-cyclodextrin and the debranching reactions of 6-O-maltooctaosyl-beta-cyclodextrin are firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced | Thermofilum pendens DSM 2475 | ? | - |
? | |
| gamma-cyclodextrin + H2O | in the initial stages, the ring-opening reactions of gamma-cyclodextrin is firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced | Thermofilum pendens | ? | - |
? | |
| pullulan + H2O | - |
Thermofilum pendens | ? | - |
? | |
| pullulan + H2O | - |
Thermofilum pendens DSM 2475 | ? | - |
? | |
| starch + H2O | - |
Thermofilum pendens | ? | - |
? | |
| starch + H2O | - |
Thermofilum pendens DSM 2475 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65500, SDS-PAGE | Thermofilum pendens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amylopullulanase | - |
Thermofilum pendens |
| TPApu | - |
Thermofilum pendens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | 100 | - |
Thermofilum pendens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
gamma-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
| 0.09 | - |
alpha-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
| 0.11 | - |
beta-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
| 0.11 | - |
amylose | pH 3.5, 95°C | Thermofilum pendens | |
| 0.73 | - |
pullulan | pH 3.5, 95°C | Thermofilum pendens | |
| 1.67 | - |
starch | pH 3.5, 95°C | Thermofilum pendens | |
| 1.69 | - |
amylopectin | pH 3.5, 95°C | Thermofilum pendens | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Thermofilum pendens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
beta-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
| 0.04 | - |
gamma-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
| 0.043 | - |
amylopectin | pH 3.5, 95°C | Thermofilum pendens | |
| 0.16 | - |
amylose | pH 3.5, 95°C | Thermofilum pendens | |
| 0.23 | - |
starch | pH 3.5, 95°C | Thermofilum pendens | |
| 0.36 | - |
alpha-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
| 8.79 | - |
pullulan | pH 3.5, 95°C | Thermofilum pendens | |
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