Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli | Thermofilum pendens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
pullulan | Km-value: 0.08 mg/ml, pH 3.5, 95°C | Thermofilum pendens | |
additional information | - |
amylose | Km-value: 0.71 mg/ml, pH 3.5, 95°C | Thermofilum pendens | |
additional information | - |
amylopectin | Km-value: 3.95 mg/ml, pH 3.5, 95°C | Thermofilum pendens | |
additional information | - |
starch | Km-value: 7.29 mg/ml, pH 3.5, 95°C | Thermofilum pendens | |
0.25 | - |
alpha-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
0.71 | - |
gamma-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
5.16 | - |
beta-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65500 | - |
SDS-PAGE | Thermofilum pendens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermofilum pendens | A1RWP0 | - |
- |
Thermofilum pendens DSM 2475 | A1RWP0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermofilum pendens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | - |
Thermofilum pendens | ? | - |
? | |
amylopectin + H2O | the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C | Thermofilum pendens | ? | - |
? | |
amylopectin + H2O | the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C | Thermofilum pendens DSM 2475 | ? | - |
? | |
amylose + H2O | - |
Thermofilum pendens | ? | - |
? | |
amylose + H2O | - |
Thermofilum pendens DSM 2475 | ? | - |
? | |
beta-cyclodextrin + H2O | in the initial stages, the ring-opening reactions of 6-O-glucosyl-beta-cyclodextrin, 6-O-maltosyl-beta-cyclodextrin and the debranching reactions of 6-O-maltooctaosyl-beta-cyclodextrin are firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced | Thermofilum pendens | ? | - |
? | |
beta-cyclodextrin + H2O | in the initial stages, the ring-opening reactions of 6-O-glucosyl-beta-cyclodextrin, 6-O-maltosyl-beta-cyclodextrin and the debranching reactions of 6-O-maltooctaosyl-beta-cyclodextrin are firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced | Thermofilum pendens DSM 2475 | ? | - |
? | |
gamma-cyclodextrin + H2O | in the initial stages, the ring-opening reactions of gamma-cyclodextrin is firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced | Thermofilum pendens | ? | - |
? | |
pullulan + H2O | - |
Thermofilum pendens | ? | - |
? | |
pullulan + H2O | - |
Thermofilum pendens DSM 2475 | ? | - |
? | |
starch + H2O | - |
Thermofilum pendens | ? | - |
? | |
starch + H2O | - |
Thermofilum pendens DSM 2475 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65500, SDS-PAGE | Thermofilum pendens |
Synonyms | Comment | Organism |
---|---|---|
amylopullulanase | - |
Thermofilum pendens |
TPApu | - |
Thermofilum pendens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | 100 | - |
Thermofilum pendens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
gamma-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
0.09 | - |
alpha-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
0.11 | - |
beta-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
0.11 | - |
amylose | pH 3.5, 95°C | Thermofilum pendens | |
0.73 | - |
pullulan | pH 3.5, 95°C | Thermofilum pendens | |
1.67 | - |
starch | pH 3.5, 95°C | Thermofilum pendens | |
1.69 | - |
amylopectin | pH 3.5, 95°C | Thermofilum pendens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Thermofilum pendens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
beta-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
0.04 | - |
gamma-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
0.043 | - |
amylopectin | pH 3.5, 95°C | Thermofilum pendens | |
0.16 | - |
amylose | pH 3.5, 95°C | Thermofilum pendens | |
0.23 | - |
starch | pH 3.5, 95°C | Thermofilum pendens | |
0.36 | - |
alpha-cyclodextrin | pH 3.5, 95°C | Thermofilum pendens | |
8.79 | - |
pullulan | pH 3.5, 95°C | Thermofilum pendens |