Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1% v/v, 149% of initial activity | Exiguobacterium sp. | |
dithiothreitol | 0.2% w/v, 147% of initial activity | Exiguobacterium sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Bacillus subtilis | Exiguobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 2 mM, 79% residual activity | Exiguobacterium sp. | |
additional information | enzyme retains 99, 89, and 54% of its activity at 10% concentration of Triton-X100, Tween 20, and SDS, respectively. The enzyme retains 80.4 and 93.7% activity in the presence of commercial detergents Rika (7.5% v/v) and Fadisheh (2.5% w/v), respectively | Exiguobacterium sp. | |
Zn2+ | 2 mM, 48% residual activity | Exiguobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
pullulan | Km value is 2.8 mg/ml, pH 8.5, 45°C | Exiguobacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2 mM, 113% of initial activity | Exiguobacterium sp. | |
Mn2+ | 2 mM, 143% of initial activity | Exiguobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
x * 110000, SDS-PAGE | Exiguobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Exiguobacterium sp. | A0A0B5JT51 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | negligible activity on wheat flour and maltodextrin, no activity on amylose | Exiguobacterium sp. | ? | - |
? | |
potato starch + H2O | 14% of the activity with pullulan | Exiguobacterium sp. | ? | - |
? | |
pullulan + H2O | - |
Exiguobacterium sp. | ? | - |
? | |
rice flour + H2O | 15% of the activity with pullulan | Exiguobacterium sp. | ? | - |
? | |
soluble starch + H2O | 17% of the activity with pullulan | Exiguobacterium sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000, SDS-PAGE | Exiguobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Exiguobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
melting temperature | Exiguobacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37 | - |
pullulan | pH 8.5, 45°C | Exiguobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Exiguobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | more than 60% of maximum activity | Exiguobacterium sp. |