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Literature summary for 3.2.1.41 extracted from
- Molecular cloning and biochemical characterization of a heat-stable type I pullulanase from Thermotoga neapolitana (2011), Enzyme Microb. Technol., 48, 260-266.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 131% of initial activity | Thermotoga neapolitana |  |
| dithiothreitol | 1 mM, 168% of initial activity | Thermotoga neapolitana | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga neapolitana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 18% residual activity | Thermotoga neapolitana | |
| Cu2+ | 1 mM, complete inactivation | Thermotoga neapolitana | |
| Hg2+ | 1 mM, complete inactivation | Thermotoga neapolitana | |
| Mn2+ | 1 mM, 15% residual activity | Thermotoga neapolitana | |
| additional information | not inhibitory: EDTA | Thermotoga neapolitana | |
| N-bromosuccinimide | complete inactivation | Thermotoga neapolitana | |
| Ni2+ | 1 mM, 18% residual activity | Thermotoga neapolitana | |
| Zn2+ | 1 mM, 53% residual activity | Thermotoga neapolitana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
x * 93000, SDS-PAGE | Thermotoga neapolitana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga neapolitana | C0LLG9 | type I enzyme | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | sequence contains a 19-residue signal peptide | Thermotoga neapolitana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.7 | - |
pH 6.5, 80°C | Thermotoga neapolitana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
| glycogen + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
| additional information | no substrate: amylose. Enzyme displays transglycosylation activity, transferring the maltotriosyl residue of pullulan to aesculin by forming alpha-1,6-glucosidic linkages | Thermotoga neapolitana | ? | - |
? | |
| pullulan + H2O | - |
Thermotoga neapolitana | maltotriose + ? | - |
? | |
| starch + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 93000, SDS-PAGE | Thermotoga neapolitana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PulA | - |
Thermotoga neapolitana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermotoga neapolitana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life 88 min | Thermotoga neapolitana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Thermotoga neapolitana |
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