Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Brij-35 | stimulates at 0.025-0.10% v/v | Bacillus acidopullulyticus | |
SDS | stimulates at 0.025-0.10% v/v | Bacillus acidopullulyticus | |
Triton X-100 | stimulates at 0.025-0.10% v/v | Bacillus acidopullulyticus | |
Tween 80 | stimulates at 0.025-0.10% v/v | Bacillus acidopullulyticus |
Application | Comment | Organism |
---|---|---|
food industry | maltotriose syrup preparation from pullulan using pullulanase, overview. In a batch system, pullulanase hydrolyzes 94.25% of purified pullulan and the resultant syrup contains 3.8 mg/ml of maltotriose | Bacillus acidopullulyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics, overview | Bacillus acidopullulyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 2.1fold | Bacillus acidopullulyticus | |
Mn2+ | activates 1.8fold | Bacillus acidopullulyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus acidopullulyticus | pullulanase is a debranching enzyme which hydrolyses the alpha-1,6-glucosidic linkages in pullulan and other amylaceous polysaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus acidopullulyticus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bacillus acidopullulyticus | - |
Storage Stability | Organism |
---|---|
purified enzyme, 6 h, 50°C, pH 5.0, completely stable | Bacillus acidopullulyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | - |
Bacillus acidopullulyticus | ? | - |
? | |
additional information | pullulanase is a debranching enzyme which hydrolyses the alpha-1,6-glucosidic linkages in pullulan and other amylaceous polysaccharides | Bacillus acidopullulyticus | ? | - |
? | |
additional information | pullulanase effectively hydrolyzes pullulan, soluble starch and dextran | Bacillus acidopullulyticus | ? | - |
? | |
pullulan + H2O | substrate from Aureobasidium pullulans strain FB-1 | Bacillus acidopullulyticus | ? | - |
? | |
soluble starch + H2O | - |
Bacillus acidopullulyticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to a family of 13 glycosyl hydrolases, also called the alpha-amylase family | Bacillus acidopullulyticus |
Pullulan 6-glucanohydrolase | - |
Bacillus acidopullulyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus acidopullulyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | - |
Bacillus acidopullulyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal inactivation kinetics, overview | Bacillus acidopullulyticus |
50 | 70 | irreversible inactivation of free pullulanase, 92.51% and 82.52% of maximal activity at 55°C and 60°C, respectively, rapid decrease at 70°C | Bacillus acidopullulyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Bacillus acidopullulyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | over 89% of maximal activity between pH 5.0 and pH 6.5, over 75% at pH 4.5 | Bacillus acidopullulyticus |