Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | at 10 mM | Pyrococcus woesei | |
dithiothreitol | at 10 mM | Pyrococcus woesei | |
Triton X-100 | at 0.1% | Pyrococcus woesei |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus woesei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cr3+ | - |
Pyrococcus woesei | |
Cu2+ | - |
Pyrococcus woesei | |
Cyclodextrins | - |
Pyrococcus woesei | |
Fe2+ | - |
Pyrococcus woesei | |
Guanidine HCl | at 0.25 M | Pyrococcus woesei | |
iodoacetamide | at 10 mM | Pyrococcus woesei | |
N-bromosuccinimide | - |
Pyrococcus woesei | |
Ni2+ | - |
Pyrococcus woesei | |
Sodium dodecyl sulfate | - |
Pyrococcus woesei | |
Urea | at 6 M | Pyrococcus woesei | |
Zn2+ | - |
Pyrococcus woesei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | highly stimulated | Pyrococcus woesei | |
Co2+ | - |
Pyrococcus woesei | |
Mn2+ | slightly stimulated | Pyrococcus woesei | |
Na2MoO4 | - |
Pyrococcus woesei | |
Na2WO4 | - |
Pyrococcus woesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel electrophoresis | Pyrococcus woesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | Pyrococcus woesei | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
pullulan + H2O | Pyrococcus woesei | - |
maltotriose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus woesei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant protein | Pyrococcus woesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Pyrococcus woesei | maltooligosaccharides + maltose | - |
? | |
amylopectin + H2O | branched polysaccharides | Pyrococcus woesei | maltooligosaccharides + maltose | - |
? | |
dextrin + H2O | - |
Pyrococcus woesei | maltooligosaccharides + maltose | - |
? | |
pullulan + H2O | - |
Pyrococcus woesei | maltotriose + ? | - |
? | |
soluble starch | - |
Pyrococcus woesei | maltotriose + maltose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amylase-pullulanase | - |
Pyrococcus woesei |
pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Pyrococcus woesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Pyrococcus woesei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 120 | 40% activity at 120°C | Pyrococcus woesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
after 4 h, 15% loss of activity | Pyrococcus woesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pyrococcus woesei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Pyrococcus woesei |