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Literature summary for 3.2.1.41 extracted from

  • Rudiger, A.; Jorgensen, P.L.; Antranikian, G.
    Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli (1995), Appl. Environ. Microbiol., 61, 567-575.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol at 10 mM Pyrococcus woesei
dithiothreitol at 10 mM Pyrococcus woesei
Triton X-100 at 0.1% Pyrococcus woesei

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus woesei

Inhibitors

Inhibitors Comment Organism Structure
Cr3+
-
Pyrococcus woesei
Cu2+
-
Pyrococcus woesei
Cyclodextrins
-
Pyrococcus woesei
Fe2+
-
Pyrococcus woesei
Guanidine HCl at 0.25 M Pyrococcus woesei
iodoacetamide at 10 mM Pyrococcus woesei
N-bromosuccinimide
-
Pyrococcus woesei
Ni2+
-
Pyrococcus woesei
Sodium dodecyl sulfate
-
Pyrococcus woesei
Urea at 6 M Pyrococcus woesei
Zn2+
-
Pyrococcus woesei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ highly stimulated Pyrococcus woesei
Co2+
-
Pyrococcus woesei
Mn2+ slightly stimulated Pyrococcus woesei
Na2MoO4
-
Pyrococcus woesei
Na2WO4
-
Pyrococcus woesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
gel electrophoresis Pyrococcus woesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylopectin + H2O Pyrococcus woesei branched polysaccharides maltooligosaccharides + maltose
-
?
pullulan + H2O Pyrococcus woesei
-
maltotriose + ?
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus woesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein Pyrococcus woesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Pyrococcus woesei maltooligosaccharides + maltose
-
?
amylopectin + H2O branched polysaccharides Pyrococcus woesei maltooligosaccharides + maltose
-
?
dextrin + H2O
-
Pyrococcus woesei maltooligosaccharides + maltose
-
?
pullulan + H2O
-
Pyrococcus woesei maltotriose + ?
-
?
soluble starch
-
Pyrococcus woesei maltotriose + maltose
-
?

Synonyms

Synonyms Comment Organism
amylase-pullulanase
-
Pyrococcus woesei
pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Pyrococcus woesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
Pyrococcus woesei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 120 40% activity at 120°C Pyrococcus woesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
after 4 h, 15% loss of activity Pyrococcus woesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pyrococcus woesei

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
Pyrococcus woesei