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Literature summary for 3.2.1.41 extracted from
- Substrate specificity and detailed characterization of a bifunctional amylase-pullulanase enzyme from Bacillus circulans F-2 having two different active sites on one polypeptide (1995), Eur. J. Biochem., 227, 687-693.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Niallia circulans |  |
| Ba2+ | - |
Niallia circulans | |
| Ca2+ | - |
Niallia circulans | |
| Cu2+ | - |
Micrococcus sp. | |
| Cu2+ | - |
Niallia circulans | |
| EDTA | - |
Micrococcus sp. | |
| EDTA | - |
Niallia circulans | |
| Fe2+ | - |
Niallia circulans | |
| Hg2+ | - |
Micrococcus sp. | |
| Hg2+ | - |
Niallia circulans | |
| Li+ | - |
Niallia circulans | |
| Mn2+ | - |
Micrococcus sp. | |
| Mn2+ | - |
Niallia circulans | |
| Pb2+ | - |
Micrococcus sp. | |
| Pb2+ | - |
Niallia circulans | |
| Sn2+ | - |
Micrococcus sp. | |
| Sn2+ | - |
Niallia circulans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | highly stimulated | Niallia circulans | |
| Co2+ | - |
Niallia circulans | |
| Ni2+ | - |
Niallia circulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 218000 | 220000 | gel filtration, gel electrophoresis, amino acid analysis | Niallia circulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Niallia circulans | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | Micrococcus sp. | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Niallia circulans | - |
maltotriose + ? | - |
? | |
| pullulan + H2O | Micrococcus sp. | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus sp. | - |
- |
- |
| Niallia circulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Niallia circulans | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | - |
Micrococcus sp. | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Niallia circulans | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Micrococcus sp. | maltooligosaccharides + maltose | - |
? | |
| amylose + H2O | - |
Niallia circulans | maltooligosaccharides + maltose | - |
? | |
| glycogen + H2O | - |
Niallia circulans | maltooligosaccharides + maltose | - |
? | |
| glycogen + H2O | - |
Micrococcus sp. | maltooligosaccharides + maltose | - |
? | |
| maltohexaose + H2O | - |
Niallia circulans | maltotriose + maltose + D-glucose + maltotetraose | - |
? | |
| maltopentaose + H2O | - |
Niallia circulans | maltotriose + maltose | - |
? | |
| maltotetraose + H2O | - |
Niallia circulans | maltose + glucose + maltotriose | - |
? | |
| pullulan + H2O | - |
Niallia circulans | maltotriose + ? | - |
? | |
| pullulan + H2O | - |
Micrococcus sp. | maltotriose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Niallia circulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amylase-pullulanase | - |
Niallia circulans |
| pullulanase type I | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan | Micrococcus sp. |
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Niallia circulans |
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