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Literature summary for 3.2.1.41 extracted from

  • Hyun, H.H.; Zeikus, J.G.
    General biochemical characterization of thermostable pullulanase and glucoamylase from Clostridium thermohydrosulfuricum (1985), Appl. Environ. Microbiol., 49, 1168-1173.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylopectin + H2O Thermoanaerobacter thermohydrosulfuricus branched polysaccharides maltooligosaccharides + maltose
-
?
pullulan + H2O Thermoanaerobacter thermohydrosulfuricus
-
maltotriose + ?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol stable in 10% Thermoanaerobacter thermohydrosulfuricus

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter thermohydrosulfuricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose
-
?
amylopectin + H2O branched polysaccharides Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose
-
?
pullulan + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltotriose + ?
-
?

Synonyms

Synonyms Comment Organism
pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Thermoanaerobacter thermohydrosulfuricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermoanaerobacter thermohydrosulfuricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Thermoanaerobacter thermohydrosulfuricus

pH Range

pH Minimum pH Maximum Comment Organism
3 8
-
Thermoanaerobacter thermohydrosulfuricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 5.5
-
Thermoanaerobacter thermohydrosulfuricus