Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Geobacillus stearothermophilus | |
EDTA | - |
Geobacillus stearothermophilus | |
Zn2+ | - |
Geobacillus stearothermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Geobacillus stearothermophilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | highly stimulated | Geobacillus stearothermophilus | |
Ca2+ | highly stimulated | Geobacillus stearothermophilus | |
Co2+ | slightly stimulated | Geobacillus stearothermophilus | |
Fe2+ | slightly stimulated | Geobacillus stearothermophilus | |
Mg2+ | slightly stimulated | Geobacillus stearothermophilus | |
Mn2+ | slightly stimulated | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
gel electrophoresis | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | Geobacillus stearothermophilus | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
pullulan + H2O | Geobacillus stearothermophilus | - |
maltotriose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant protein | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
49.6 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Geobacillus stearothermophilus | maltooligosaccharides + maltose | - |
? | |
amylopectin + H2O | branched polysaccharides | Geobacillus stearothermophilus | maltooligosaccharides + maltose | - |
? | |
pullulan + H2O | - |
Geobacillus stearothermophilus | maltotriose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 83000, gel electrophoresis | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Geobacillus stearothermophilus |