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Literature summary for 3.2.1.41 extracted from
- Cell-associated pullulanase from Bacteroides thetaiotaomicron: cloning, characterization, and insertional mutagenesis to determine role in pullulan utilization (1989), J. Bacteriol., 171, 2116-2123.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacteroides thetaiotaomicron |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | 80% soluble | Bacteroides thetaiotaomicron | 5737 | - |
| membrane | 18% membrane associated | Bacteroides thetaiotaomicron | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
gel filtration | Bacteroides thetaiotaomicron |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Bacteroides thetaiotaomicron | branched polysaccharides | maltooligosaccharides + maltose | - |
? |  |
| pullulan + H2O | Bacteroides thetaiotaomicron | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides thetaiotaomicron | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein | Bacteroides thetaiotaomicron |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Bacteroides thetaiotaomicron | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Bacteroides thetaiotaomicron | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | - |
Bacteroides thetaiotaomicron | maltotriose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 77000, gel electrophoresis | Bacteroides thetaiotaomicron |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Bacteroides thetaiotaomicron |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bacteroides thetaiotaomicron |
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