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Literature summary for 3.2.1.40 extracted from
- Purification and characterization of an intracellular alpha-L-rhamnosidase from a newly isolated strain, Alternaria alternata SK37.001 (2018), Food Chem., 269, 63-69 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme is used to enhance wine aromas or to debitter citrus juices by releasing L-rhamnose | Alternaria alternata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 2 mM, 36% loss of activity | Alternaria alternata |  |
| Cu2+ | 2 mM, 53% loss of activity | Alternaria alternata | |
| Mg2+ | 2 mM, slight inhibition | Alternaria alternata | |
| Mn2+ | 2 mM, slight inhibition | Alternaria alternata | |
| Ni2+ | 2 mM, complete loss of activity | Alternaria alternata | |
| Zn2+ | 2 mM, slight inhibition | Alternaria alternata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.16 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 5.5, 50°C | Alternaria alternata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Alternaria alternata | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 2 mM, 1.25fold activation | Alternaria alternata | |
| Ba2+ | 2 mM, 1.35fold activation | Alternaria alternata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
SDS-PAGE | Alternaria alternata |
| 135000 | - |
gel filtration | Alternaria alternata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alternaria alternata | - |
- |
- |
| Alternaria alternata SK37.001 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alternaria alternata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Alternaria alternata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.7 | - |
purified enzyme, pH 5.5, 50°C | Alternaria alternata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-rhamnopyranoside + H2O | - |
Alternaria alternata | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
| 4-nitrophenyl alpha-L-rhamnopyranoside + H2O | - |
Alternaria alternata SK37.001 | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
| hesperidin + H2O | - |
Alternaria alternata | hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose | - |
? | |
| additional information | the enzyme can not hydrolyze ginsenoside Rg2 or saikosaponin C | Alternaria alternata | ? | - |
? | |
| additional information | the enzyme can not hydrolyze ginsenoside Rg2 or saikosaponin C | Alternaria alternata SK37.001 | ? | - |
? | |
| naringin + H2O | - |
Alternaria alternata | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
| naringin + H2O | - |
Alternaria alternata SK37.001 | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
| neohesperidin + H2O | - |
Alternaria alternata | 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside + alpha-L-rhamnose | - |
? | |
| neohesperidin + H2O | - |
Alternaria alternata SK37.001 | 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside + alpha-L-rhamnose | - |
? | |
| quercitrin + H2O | - |
Alternaria alternata | quercetin + alpha-L-rhamnose | - |
? | |
| rutin + H2O | - |
Alternaria alternata | isoquercitrin + alpha-L-rhamnose | - |
? | |
| rutin + H2O | - |
Alternaria alternata SK37.001 | isoquercitrin + alpha-L-rhamnose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 66000, SDS-PAGE | Alternaria alternata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Alternaria alternata |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | 40°C: about 55% of maximal activity, 70°C: about 30% of maximal activity | Alternaria alternata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 4 h, residual enzyme activity is more than 60% | Alternaria alternata |
| 50 | - |
t1/2: 630 min | Alternaria alternata |
| 55 | - |
t1/2: 240 min | Alternaria alternata |
| 60 | - |
t1/2: 59.5 min | Alternaria alternata |
| 65 | - |
t1/2: 45.9 min | Alternaria alternata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
substrate: 4-nitrophenyl alpha-L-rhamnopyranoside | Alternaria alternata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | 12 h, 4°C, most of its activity is retained | Alternaria alternata |
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