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Literature summary for 3.2.1.40 extracted from

  • Yu, H.; Liu, H.; Zhang, C.; Tan, D.; Lu, M.; Jin, F.
    Purification and characterization of gypenoside-alpha-L-rhamnosidase hydrolyzing gypenoside-5 into ginsenoside Rd (2004), Process Biochem., 39, 861-867.
No PubMed abstract available
Search for more literature for 3.2.1.40

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ activates 1.4fold at 20 mM, inhibits completely at 100 mM Absidia sp. (in: Fungi)
Fe3+ activates 1.4fold at 10 mM, inhibits completely at 50 mM Absidia sp. (in: Fungi)

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 1.8fold at 10 mM Absidia sp. (in: Fungi)
Mg2+ activates 2fold at 10 mM Absidia sp. (in: Fungi)
Zn2+ activates 3fold at 10 mM Absidia sp. (in: Fungi)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
gypenoside-5 + H2O Absidia sp. (in: Fungi)
-
 ginsenoside Rd + alpha-L-rhamnose
-
 ?

Organism

Organism UniProt Comment Textmining
Absidia sp. (in: Fungi)
-
 strain 90, FFDCL-90, and strain 39, FFDCL-39, isolated from traditional Chinese Koji
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain FFDCL-90, by ammonium sulfate fractionation and ion exchange chromatography Absidia sp. (in: Fungi)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.44
-
 purified enzyme Absidia sp. (in: Fungi)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
20(S)-ginsenoside Rg2 + H2O low activity cleaving the alpha-1,2-linkage Absidia sp. (in: Fungi) 20(S)-ginsenoside Rh1 + alpha-L-rhamnose
-
 ?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Absidia sp. (in: Fungi) 4-nitrophenol + alpha-L-rhamnose
-
 ?
gypenoside-5 + H2O
-
 Absidia sp. (in: Fungi) ginsenoside Rd + alpha-L-rhamnose
-
 ?
gypenoside-5 + H2O cleavage of the alpha-1,6-linkage Absidia sp. (in: Fungi) ginsenoside Rd + alpha-L-rhamnose
-
 ?

Synonyms

Synonyms Comment Organism
gypenoside-alpha-L-rhamnosidase
-
 Absidia sp. (in: Fungi)
saponin-alpha-L-rhamnosidase
-
 Absidia sp. (in: Fungi)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Absidia sp. (in: Fungi)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 55% of maximal activity at 30°C, 40% at 60°C Absidia sp. (in: Fungi)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Absidia sp. (in: Fungi)

pH Range

pH Minimum pH Maximum Comment Organism
2 10 40% of maximal activity at pH 4.0, 10% at pH 10.0 Absidia sp. (in: Fungi)

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 purified enzyme, stable Absidia sp. (in: Fungi)