BRENDA - Enzyme Database show
show all sequences of 3.2.1.40

Two new thermostable alpha-L-rhamnosidases from a novel thermophilic bacterium

Birgisson, H.; Hreggvidsson, G.O.; Fridjonsson, O.H.; Mort, A.; Kristjansson, J.K.; Mattiasson, B.; Enzyme Microb. Technol. 34, 561-571 (2004)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
alpha-L-rhamnosidases are used in wine production; alpha-L-rhamnosidases are used in wine production
Thermomicrobia bacterium
Cloned(Commentary)
Commentary
Organism
gene rhmA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli; gene rhmB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
Thermomicrobia bacterium
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-glucose
about 500 mM D-glucose concentration in grape juice inhibits RhmA by 39%; about 500 mM D-glucose concentration in grape juice inhibits RhmB by 38%
Thermomicrobia bacterium
ethanol
at 12% v/v, 51% inhibition of RhmB; at 12% v/v, 60% inhibition of RhmA
Thermomicrobia bacterium
L-rhamnose
complete inhibition, competitive product inhibition; complete inhibition, competitive product inhibition
Thermomicrobia bacterium
Zn2+
30-35% inhibition at 2 mM, reversible by 10 mM EDTA
Thermomicrobia bacterium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics; kinetics
Thermomicrobia bacterium
0.46
-
4-nitrophenyl alpha-L-rhamnopyranoside
pH 7.5, 50°C, recombinant RhmA
Thermomicrobia bacterium
0.66
-
4-nitrophenyl alpha-L-rhamnopyranoside
pH 7.0, 50°C, recombinant RhmB
Thermomicrobia bacterium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no effect by EDTA, Mg2+, Ca2+, and Mn2+ on RhmB; no effect by EDTA, Zn2+, Mg2+, Ca2+, and Mn2+ on RhmA
Thermomicrobia bacterium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
104000
-
2 * 104000, recombinant enzyme, SDS-PAGE
Thermomicrobia bacterium
107000
-
2 * 107000, recombinant enzyme, SDS-PAGE
Thermomicrobia bacterium
210000
-
recombinant enzyme, gel filtration; recombinant enzyme, gel filtration
Thermomicrobia bacterium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermomicrobia bacterium
Q6RCI8
gene rhmB, isozyme RhmB
-
Thermomicrobia bacterium
Q6RCI9
gene rhmA, isozyme RhmA
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme 63fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography; recombinant enzyme 85fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography
Thermomicrobia bacterium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
108.9
-
purified recombinant enzyme
Thermomicrobia bacterium
263.9
-
purified recombinant enzyme
Thermomicrobia bacterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
664775
Thermomicrobia bacterium
4-nitrophenol + alpha-L-rhamnose
-
-
-
?
hesperidin + H2O
cleavage of the alpha-1,6-linkage
664775
Thermomicrobia bacterium
? + alpha-L-rhamnose
-
-
-
?
additional information
no activity with quercitrin
664775
Thermomicrobia bacterium
?
-
-
-
-
additional information
no activity with rutin and quercitrin
664775
Thermomicrobia bacterium
?
-
-
-
-
naringin + H2O
preferred substrate, cleavage of the alpha-1,2-linkage
664775
Thermomicrobia bacterium
? + alpha-L-rhamnose
-
-
-
?
rhamnogalacturonan tetramer + H2O
-
664775
Thermomicrobia bacterium
?
-
-
-
?
rutin + H2O
low activity, cleavage of the alpha-1,6-linkage
664775
Thermomicrobia bacterium
? + alpha-L-rhamnose
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 104000, recombinant enzyme, SDS-PAGE; 2 * 107000, recombinant enzyme, SDS-PAGE
Thermomicrobia bacterium
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
;
Thermomicrobia bacterium
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
80
low activity above; low activity above
Thermomicrobia bacterium
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
completely stable for 24 h at pH 5.0-6.9; loss of 22% activity within 24 h at pH 7.9
Thermomicrobia bacterium
60
-
20-30% remaining activity after 24 h at pH 5.0-6.9; 20-30% remaining activity after 24 h at pH 7.9
Thermomicrobia bacterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6.9
-
Thermomicrobia bacterium
7.9
-
-
Thermomicrobia bacterium
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
7.9
50% of maximal activity at pH 4.0 and pH 7.9
Thermomicrobia bacterium
5
8.7
50% of maximal activity at pH 5.0 and pH 8.7
Thermomicrobia bacterium
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.28
-
L-rhamnose
pH 7.5, 50°C, recombinant RhmA, versus 4-nitrophenyl alpha-L-rhamnopyranoside
Thermomicrobia bacterium
4.65
-
L-rhamnose
pH 7.0, 50°C, recombinant RhmB, versus 4-nitrophenyl alpha-L-rhamnopyranoside
Thermomicrobia bacterium
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Thermomicrobia bacterium
sequence calculation
-
4.5
Thermomicrobia bacterium
sequence calculation
-
4.6
Application (protein specific)
Application
Commentary
Organism
nutrition
alpha-L-rhamnosidases are used in wine production
Thermomicrobia bacterium
Cloned(Commentary) (protein specific)
Commentary
Organism
gene rhmA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
Thermomicrobia bacterium
gene rhmB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
Thermomicrobia bacterium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-glucose
about 500 mM D-glucose concentration in grape juice inhibits RhmA by 39%
Thermomicrobia bacterium
D-glucose
about 500 mM D-glucose concentration in grape juice inhibits RhmB by 38%
Thermomicrobia bacterium
ethanol
at 12% v/v, 60% inhibition of RhmA
Thermomicrobia bacterium
ethanol
at 12% v/v, 51% inhibition of RhmB
Thermomicrobia bacterium
L-rhamnose
complete inhibition, competitive product inhibition
Thermomicrobia bacterium
Zn2+
30-35% inhibition at 2 mM, reversible by 10 mM EDTA
Thermomicrobia bacterium
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.28
-
L-rhamnose
pH 7.5, 50°C, recombinant RhmA, versus 4-nitrophenyl alpha-L-rhamnopyranoside
Thermomicrobia bacterium
4.65
-
L-rhamnose
pH 7.0, 50°C, recombinant RhmB, versus 4-nitrophenyl alpha-L-rhamnopyranoside
Thermomicrobia bacterium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Thermomicrobia bacterium
0.46
-
4-nitrophenyl alpha-L-rhamnopyranoside
pH 7.5, 50°C, recombinant RhmA
Thermomicrobia bacterium
0.66
-
4-nitrophenyl alpha-L-rhamnopyranoside
pH 7.0, 50°C, recombinant RhmB
Thermomicrobia bacterium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no effect by EDTA, Zn2+, Mg2+, Ca2+, and Mn2+ on RhmA
Thermomicrobia bacterium
additional information
no effect by EDTA, Mg2+, Ca2+, and Mn2+ on RhmB
Thermomicrobia bacterium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
104000
-
2 * 104000, recombinant enzyme, SDS-PAGE
Thermomicrobia bacterium
107000
-
2 * 107000, recombinant enzyme, SDS-PAGE
Thermomicrobia bacterium
210000
-
recombinant enzyme, gel filtration
Thermomicrobia bacterium
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme 63fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography
Thermomicrobia bacterium
recombinant enzyme 85fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography
Thermomicrobia bacterium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
108.9
-
purified recombinant enzyme
Thermomicrobia bacterium
263.9
-
purified recombinant enzyme
Thermomicrobia bacterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
664775
Thermomicrobia bacterium
4-nitrophenol + alpha-L-rhamnose
-
-
-
?
hesperidin + H2O
cleavage of the alpha-1,6-linkage
664775
Thermomicrobia bacterium
? + alpha-L-rhamnose
-
-
-
?
additional information
no activity with quercitrin
664775
Thermomicrobia bacterium
?
-
-
-
-
additional information
no activity with rutin and quercitrin
664775
Thermomicrobia bacterium
?
-
-
-
-
naringin + H2O
preferred substrate, cleavage of the alpha-1,2-linkage
664775
Thermomicrobia bacterium
? + alpha-L-rhamnose
-
-
-
?
rhamnogalacturonan tetramer + H2O
-
664775
Thermomicrobia bacterium
?
-
-
-
?
rutin + H2O
low activity, cleavage of the alpha-1,6-linkage
664775
Thermomicrobia bacterium
? + alpha-L-rhamnose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 104000, recombinant enzyme, SDS-PAGE
Thermomicrobia bacterium
dimer
2 * 107000, recombinant enzyme, SDS-PAGE
Thermomicrobia bacterium
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Thermomicrobia bacterium
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
80
low activity above
Thermomicrobia bacterium
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
loss of 22% activity within 24 h at pH 7.9
Thermomicrobia bacterium
40
-
completely stable for 24 h at pH 5.0-6.9
Thermomicrobia bacterium
60
-
20-30% remaining activity after 24 h at pH 7.9
Thermomicrobia bacterium
60
-
20-30% remaining activity after 24 h at pH 5.0-6.9
Thermomicrobia bacterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6.9
-
Thermomicrobia bacterium
7.9
-
-
Thermomicrobia bacterium
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
7.9
50% of maximal activity at pH 4.0 and pH 7.9
Thermomicrobia bacterium
5
8.7
50% of maximal activity at pH 5.0 and pH 8.7
Thermomicrobia bacterium
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Thermomicrobia bacterium
sequence calculation
-
4.5
Thermomicrobia bacterium
sequence calculation
-
4.6
Other publictions for EC 3.2.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749479
Guillotin
-
Biochemical characterization ...
Dictyoglomus thermophilum, Dictyoglomus thermophilum ATCC 35947
ACS Omega
4
1916-1922
2019
-
-
1
-
-
-
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
2
-
1
1
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
2
-
1
1
-
1
1
-
-
-
-
-
-
-
1
1
750025
Kumar
An alkali tolerant alpha-L-rh ...
Fusarium verticillioides, Fusarium verticillioides MTCC-2088
Bioorg. Chem.
84
24-31
2019
-
1
-
-
-
-
-
1
1
-
1
-
-
2
-
-
1
-
-
-
-
-
8
1
1
-
7
1
2
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
-
-
8
1
1
-
7
1
2
-
1
-
-
-
-
-
1
1
750899
Li
Heterologous expression and c ...
Aspergillus tubingensis, Aspergillus tubingensis JMU-TS529
J. Agric. Food Chem.
67
2926-2935
2019
-
1
1
-
-
-
1
1
-
-
1
-
-
2
-
1
1
-
-
-
-
-
2
1
1
-
-
1
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
-
1
-
-
-
1
1
-
-
-
-
2
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
749441
Li
Characterization of a glycosi ...
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron VPI-5482
3 Biotech
8
120
2018
-
1
1
-
12
-
8
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
2
1
1
1
1
1
1
1
3
-
-
-
-
-
1
1
-
-
12
-
-
8
-
1
-
-
1
-
-
-
-
1
-
-
-
-
2
1
1
1
1
1
1
1
3
-
-
-
-
-
1
1
749499
Pachl
Crystal structure of native a ...
Aspergillus terreus, Aspergillus terreus CCF 3059
Acta Crystallogr. Sect. D
74
1078-1084
2018
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
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1
-
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1
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1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749745
Mensitieri
Structural and functional ins ...
Novosphingobium sp. PP1Y
Arch. Biochem. Biophys.
648
1-11
2018
-
-
1
-
5
-
-
7
-
2
1
-
-
3
-
-
1
-
-
-
-
-
6
1
1
-
-
7
1
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
7
-
2
1
-
-
-
-
1
-
-
-
-
6
1
1
-
-
7
1
-
-
-
-
-
-
-
7
7
750024
Wu
Characterization of a alpha-L ...
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron VPI-5482
Bioorg. Chem.
81
461-467
2018
-
-
1
-
-
-
6
1
-
2
1
-
-
5
-
-
1
-
-
-
-
-
10
1
1
2
2
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
6
-
1
-
2
1
-
-
-
-
1
-
-
-
-
10
1
1
2
2
1
1
-
1
-
1
-
-
1
-
-
750586
Li
Development and characterizat ...
Aspergillus niger, Aspergillus niger JMU-TS528
Food Chem.
245
1070-1078
2018
-
1
1
-
1
-
-
2
-
-
-
-
-
5
-
-
-
-
-
-
-
-
12
-
-
-
3
2
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
3
2
-
-
-
-
-
-
-
-
2
2
750590
Zhang
Purification and characteriza ...
Alternaria alternata, Alternaria alternata SK37.001
Food Chem.
269
63-69
2018
-
1
-
-
-
-
6
1
1
2
2
-
-
2
-
-
1
-
-
1
1
-
12
1
1
1
5
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
6
-
1
1
2
2
-
-
-
-
1
-
1
1
-
12
1
1
1
5
-
1
-
1
-
-
-
-
-
-
-
750785
Li
Improving the thermostability ...
Aspergillus niger, Aspergillus niger JMU-TS528
Int. J. Biol. Macromol.
112
14-21
2018
-
1
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
1
-
-
-
-
-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
750855
Lei
-
Enzymatic production of natur ...
Aspergillus niger, Aspergillus niger JMU-TS528
Int. J. Food Sci. Technol.
53
2097-2103
2018
-
1
-
-
-
1
3
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
1
1
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-
751161
Ge
B-factor-saturation mutagenes ...
Aspergillus terreus
J. Biotechnol.
275
17-23
2018
-
2
-
-
8
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
3
-
2
-
1
1
1
3
1
1
-
-
-
-
-
-
2
-
-
-
8
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
2
-
1
1
1
3
1
1
-
-
-
-
-
-
3
3
750193
Ge
Enhancing the thermostability ...
Aspergillus terreus
BMC Biotechnol.
17
21
2017
-
2
1
-
-
-
-
3
-
-
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Cui
Crystallization and preliminar ...
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Miyata
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664775
Birgisson
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Two new thermostable alpha-L-r ...
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Purification and characterizat ...
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655015
Soria
Thermal inactivation and produ ...
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656674
Scaroni
Hydrolytic properties of crude ...
Aspergillus flavus, Aspergillus flavus S1, Aspergillus kawachii, Aspergillus kawachii IFO 4308, Fusarium sambucinum, Fusarium sambucinum 310, Fusarium solani, Fusarium solani 706, Mucor racemosus, Mucor racemosus 709, Penicillium aurantiogriseum, Penicillium aurantiogriseum 695, Trichoderma longibrachiatum, Trichoderma longibrachiatum 708
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1
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14
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6
1
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6
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654256
Manzanares
Purification and characterizat ...
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1
1
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6
2
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Purification and characterizat ...
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Manzanares
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alpha-L-Rhamnosidase from Fago ...
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Bourbouze
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Fagopyrum esculentum
Phytochemistry
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alpha-L-Rhamnosidase activity ...
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Kurosawa
alpha-L-Rhamnosidases of the l ...
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