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Literature summary for 3.2.1.40 extracted from
- Purification and characterization of an alpha-L-rhamnosidase from Pichia angusta X349 (2000), Biosci. Biotechnol. Biochem., 64, 2179-2185.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | enzyme efficiently releases monoterpenols from an aroma precursor from muscat grape juice | Ogataea angusta |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuCl2 | 1 mM, 11% residual activity | Ogataea angusta |  |
| D-glucose | 500 mM, 76% residual activity | Ogataea angusta | |
| ethanol | 12% v/v, 20% inhibition | Ogataea angusta | |
| HgCl2 | 1 mM, 11% residual activity | Ogataea angusta | |
| L-rhamnose | competitive | Ogataea angusta | |
| additional information | not inhibitory at 1 mM: EDTA, CaCl2, 2-mercaptoethanol | Ogataea angusta | |
| p-chloromercuribenzoate | 1 mM, 11% residual activity | Ogataea angusta | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Ogataea angusta | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
1 * 88000, SDS-PAGE | Ogataea angusta |
| 90000 | - |
PAGE | Ogataea angusta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ogataea angusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Ogataea angusta | 4-nitrophenol + D-glucopyranose | 2.1% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside | ? | |
| hesperidin + H2O | 34% of activity compared to naringin | Ogataea angusta | ? | - |
? | |
| muscat glycoside extract | - |
Ogataea angusta | linalool + alpha-terpineol + citronellol + nerol + geraniol + ? | - |
? | |
| naringin + H2O | - |
Ogataea angusta | ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Ogataea angusta | p-nitrophenol + alpha-L-arabinofuranose | 6.1% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside | ? | |
| p-nitrophenyl-alpha-L-rhamnopyranoside + H2O | - |
Ogataea angusta | p-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
| quercetin 3-alpha-L-rhamnopyranoside + H2O | 31% of activity compared to naringin | Ogataea angusta | quercetin + L-rhamnose | - |
? | |
| rutin + H2O | 63% of activity compared to naringin | Ogataea angusta | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 88000, SDS-PAGE | Ogataea angusta |
| More | N-terminal sequence | Ogataea angusta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Ogataea angusta |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below | Ogataea angusta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Ogataea angusta |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Ogataea angusta |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 5 | L-rhamnose | pH 6.5, 30°C | Ogataea angusta | |
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